000112061 001__ 112061
000112061 005__ 20240319080953.0
000112061 0247_ $$2doi$$a10.3389/fmicb.2022.767912
000112061 0248_ $$2sideral$$a127909
000112061 037__ $$aART-2022-127909
000112061 041__ $$adeu
000112061 100__ $$aComín, Jessica
000112061 245__ $$aIn-depth Analysis of IS6110 Genomic Variability in the Mycobacterium tuberculosis Complex
000112061 260__ $$c2022
000112061 5060_ $$aAccess copy available to the general public$$fUnrestricted
000112061 5203_ $$aThe insertion sequence (IS) 6110 is a repetitive mobile element specific for the Mycobacterium tuberculosis complex (MTBC) used for years to diagnose and genotype this pathogen. It contains the overlapping reading frames orfA and orfB that encode a transposase. Its genetic variability is difficult to study because multiple copies are present in the genome. IS6110 is randomly located, nevertheless some preferential locations have been reported, which could be related to the behaviour of the strains. The aim of this work was to determine the intra- and inter-strain genetic conservation of this element in the MTBC. For this purpose, we analysed 158 sequences of IS6110 copies from 55 strains. Eighty-four copies were from 17 strains for which we knew all the locations in their genome. In addition, we studied 74 IS6110 copies in 38 different MTBC strains in which the location was characteristic of different families including Haarlem, LAM, S, and L6 strains. We observed mutation in 13.3% of the copies studied and we found 10 IS6110 variants in 21 copies belonging to 16 strains. The high copy number strains showed 6.2% of their IS6110 copies mutated, in contrast with the 31.1% in the low-copy-number strains. The apparently more ancient copy localised in the DR region was that with more variant copies, probably because this was the most studied location. Notably, all Haarlem and X family strains studied have an IS6110 in Rv0403c, suggesting a common origin for both families. Nevertheless, we detected a variant specific for the X family that would have occurred in this location after the phylogenetic separation. This variant does not prevent transposition although it may occur at a lower frequency, as X strains remain with low copy number (LCN) of IS6110.
000112061 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000112061 590__ $$a5.2$$b2022
000112061 592__ $$a1.19$$b2022
000112061 591__ $$aMICROBIOLOGY$$b38 / 135 = 0.281$$c2022$$dQ2$$eT1
000112061 593__ $$aMicrobiology (medical)$$c2022$$dQ1
000112061 593__ $$aMicrobiology$$c2022$$dQ1
000112061 594__ $$a7.8$$b2022
000112061 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000112061 700__ $$0(orcid)0000-0003-1027-7583$$aOtal, Isabel$$uUniversidad de Zaragoza
000112061 700__ $$0(orcid)0000-0002-3011-2519$$aSamper, Sofía$$uUniversidad de Zaragoza
000112061 7102_ $$11011$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Microbiología
000112061 773__ $$g13, 767912 (2022), [11 pp]$$pFront. microbiol.$$tFrontiers in Microbiology$$x1664-302X
000112061 8564_ $$s2132732$$uhttps://zaguan.unizar.es/record/112061/files/texto_completo.pdf$$yVersión publicada
000112061 8564_ $$s2198179$$uhttps://zaguan.unizar.es/record/112061/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000112061 909CO $$ooai:zaguan.unizar.es:112061$$particulos$$pdriver
000112061 951__ $$a2024-03-18-13:17:52
000112061 980__ $$aARTICLE