Comparison of circulating CD4+, CD8+ lymphocytes and cytokine profiles between dogs with atopic dermatitis and healthy dogs
Verde, María Teresa (Universidad de Zaragoza) ; Villanueva-Saz, Sergio (Universidad de Zaragoza) ; Loste, Araceli (Universidad de Zaragoza) ; Marteles, Diana ; Pereboom, Desirée (Universidad de Zaragoza) ; Conde, Tomás (Universidad de Zaragoza) ; Fernández, Antonio
Resumen: Introduction
Canine atopic dermatitis (cAD) is an inflammatory skin disease characterized by impaired immune function. Changes in the proportions of CD4+ and CD8+ lymphocytes and the serum concentrations of cytokines in the pathogenesis of cAD have been described.
Objectives
To assess whether the changes in the ratio of CD4+ and CD8+ lymphocytes in the peripheral blood of atopic dogs at the time of diagnosis are related to the severity of the disease. Furthermore, we determined whether the changes in the serum concentrations of the cytokines IL-31, IL-34, IL-10, IFN-γ, and TNF-α were different between atopic and control dogs.
Procedures
Fifty-six client-owned dogs with atopic dermatitis and 53 healthy control dogs were used. The percentages of CD4+ and CD8+ lymphocytes were determined by imaging flow cytometry. The index of CADESI-03 was calculated. Serum cytokine levels were analyzed using ELISA.
Results
Atopic dogs showed a higher percentage of CD8+ lymphocytes, a lower CD4+/CD8+ ratio than healthy dogs, and a positive correlation with CADESI-03. Atopic dogs also showed higher serum IL-31 and IL-34 levels and lower IL-10 levels. A moderate positive correlation was found between serum IL-31 and CADESI-03.
Conclusions
The CD4+/CD8+ ratio may be a sensitive parameter that positively correlates with the severity of cAD, and elevated serum levels of IL-31 and IL-34 may facilitate diagnosis of the disease.
Idioma: Inglés
DOI: 10.1016/j.rvsc.2022.01.018
Año: 2022
Publicado en: RESEARCH IN VETERINARY SCIENCE 145 (2022), 13-20
ISSN: 0034-5288
Factor impacto JCR: 2.4 (2022)
Categ. JCR: VETERINARY SCIENCES rank: 29 / 144 = 0.201 (2022) - Q1 - T1
Factor impacto CITESCORE: 4.1 - Veterinary (Q1)
Factor impacto SCIMAGO: 0.577 - Veterinary (miscellaneous) (Q1)
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
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Exportado de SIDERAL (2024-03-18-13:41:00)
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Canine atopic dermatitis (cAD) is an inflammatory skin disease characterized by impaired immune function. Changes in the proportions of CD4+ and CD8+ lymphocytes and the serum concentrations of cytokines in the pathogenesis of cAD have been described.
Objectives
To assess whether the changes in the ratio of CD4+ and CD8+ lymphocytes in the peripheral blood of atopic dogs at the time of diagnosis are related to the severity of the disease. Furthermore, we determined whether the changes in the serum concentrations of the cytokines IL-31, IL-34, IL-10, IFN-γ, and TNF-α were different between atopic and control dogs.
Procedures
Fifty-six client-owned dogs with atopic dermatitis and 53 healthy control dogs were used. The percentages of CD4+ and CD8+ lymphocytes were determined by imaging flow cytometry. The index of CADESI-03 was calculated. Serum cytokine levels were analyzed using ELISA.
Results
Atopic dogs showed a higher percentage of CD8+ lymphocytes, a lower CD4+/CD8+ ratio than healthy dogs, and a positive correlation with CADESI-03. Atopic dogs also showed higher serum IL-31 and IL-34 levels and lower IL-10 levels. A moderate positive correlation was found between serum IL-31 and CADESI-03.
Conclusions
The CD4+/CD8+ ratio may be a sensitive parameter that positively correlates with the severity of cAD, and elevated serum levels of IL-31 and IL-34 may facilitate diagnosis of the disease.
Idioma: Inglés
DOI: 10.1016/j.rvsc.2022.01.018
Año: 2022
Publicado en: RESEARCH IN VETERINARY SCIENCE 145 (2022), 13-20
ISSN: 0034-5288
Factor impacto JCR: 2.4 (2022)
Categ. JCR: VETERINARY SCIENCES rank: 29 / 144 = 0.201 (2022) - Q1 - T1
Factor impacto CITESCORE: 4.1 - Veterinary (Q1)
Factor impacto SCIMAGO: 0.577 - Veterinary (miscellaneous) (Q1)
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.Exportado de SIDERAL (2024-03-18-13:41:00)
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Registro creado el 2022-12-22, última modificación el 2024-03-19