Engineering bio-brick protein scaffolds for organizing enzyme assemblies

Ledesma-Fernandez, Alba ; Velasco-Lozano, Susana ; Campos-Muelas, Pedro ; Madrid, Ricardo ; López-Gallego, Fernando ; Cortajarena, Aitziber L.

Resumen: Enzyme scaffolding is an emerging approach for enhancing the catalytic efficiency of multi‐enzymatic cascades by controlling their spatial organization and stoichiometry. This study introduces a novel family of engineered SCAffolding Bricks, named SCABs, utilizing the consensus tetratricopeptide repeat (CTPR) domain for organized multi‐enzyme systems. Two SCAB systems are developed, one employing head‐to‐tail interactions with reversible covalent disulfide bonds, the other relying on non‐covalent metal‐driven assembly via engineered metal coordinating interfaces. Enzymes are directly fused to SCAB modules, triggering assembly in a non‐reducing environment or by metal presence. A proof‐of‐concept with formate dehydrogenase (FDH) and L‐alanine dehydrogenase (AlaDH) shows enhanced specific productivity by 3.6‐fold compared to free enzymes, with the covalent stapling outperforming the metal‐driven assembly. This enhancement likely stems from higher‐order supramolecular assembly and improved NADH cofactor regeneration, resulting in more efficient cascades. This study underscores the potential of protein engineering to tailor scaffolds, leveraging supramolecular spatial‐organizing tools, for more efficient enzymatic cascade reactions.

Idioma: Inglés

DOI: 10.1002/pro.4984

Año: 2024

Publicado en: Protein science 33, 5 (2024), e4984 [17 pp.]
ISSN: 0961-8368

Factor impacto JCR: 5.2 (2024)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 68 / 320 = 0.213 (2024) - Q1 - T1

Factor impacto CITESCORE: 12.4 - Biochemistry (Q1) - Molecular Biology (Q1)

Factor impacto SCIMAGO: 4.657 - Biochemistry (Q1) - Molecular Biology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2019-111649RB-I00
Financiación: info:eu-repo/grantAgreement/EC/Era-CoBiotech/HOMBIOCAT/PCI2018-092984
Financiación: info:eu-repo/grantAgreement/ES/MICINN AEI/PID2022-137977OB-I00

Tipo y forma: Artículo (Versión definitiva)