000048112 001__ 48112
000048112 005__ 20180222094943.0
000048112 0247_ $$2doi$$a10.1099/jgv.0.000292
000048112 0248_ $$2sideral$$a93736
000048112 037__ $$aART-2015-93736
000048112 041__ $$aeng
000048112 100__ $$0(orcid)0000-0002-8692-1382$$aMediano, Diego R.
000048112 245__ $$aCharacterization of mesenchymal stem cells in sheep naturally infected with scrapie
000048112 260__ $$c2015
000048112 5060_ $$aAccess copy available to the general public$$fUnrestricted
000048112 5203_ $$aMesenchymal stem cells (MSCs) can be infected with prions and have been proposed as in vitro cell-based models for prion replication. In addition, autologous MSCs are of interest for cell therapy in neurodegenerative diseases. To the best of our knowledge, the effect of prion diseases on the characteristics of these cells has never been investigated. Here, we analysed the properties of MSCs obtained from bone marrow (BM-MSCs) and peripheral blood (PB-MSCs) of sheep naturally infected with scrapie — a large mammal model for the study of prion diseases. After three passages of expansion, MSCs derived from scrapie animals displayed similar adipogenic, chondrogenic and osteogenic differentiation ability as cells from healthy controls, although a subtle decrease in the proliferation potential was observed. Exceptionally, mesenchymal markers such as CD29 were significantly upregulated at the transcript level compared with controls. Scrapie MSCs were able to transdifferentiate into neuron-like cells, but displayed lower levels of neurogenic markers at basal conditions, which could limit this potential. The expression levels of cellular prion protein (PrPC) were highly variable between cultures, and no significant differences were observed between control and scrapie-derived MSCs. However, during neurogenic differentiation the expression of PrPC was upregulated in MSCs. This characteristic could be useful for developing in vitro models for prion replication. Despite the infectivity reported for MSCs obtained from scrapie-infected mice and Creutzfeldt–Jakob disease patients, protein misfolding cyclic amplification did not detect PrPSc in BM- or PB-MSCs from scrapie-infected sheep, which limits their use for in vivo diagnosis for scrapie.
000048112 536__ $$9info:eu-repo/grantAgreement/ES/UZ/2012-BIO-03$$9info:eu-repo/grantAgreement/ES/DGA/CTP2013-P05$$9info:eu-repo/grantAgreement/ES/DGA/CTPP2-13
000048112 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000048112 590__ $$a3.192$$b2015
000048112 591__ $$aVIROLOGY$$b13 / 33 = 0.394$$c2015$$dQ2$$eT2
000048112 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b46 / 161 = 0.286$$c2015$$dQ2$$eT1
000048112 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000048112 700__ $$aSanz-Rubio, David
000048112 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000048112 700__ $$0(orcid)0000-0002-1590-3347$$aMarín, Belén
000048112 700__ $$0(orcid)0000-0002-8712-2275$$aVázquez, Francisco J.$$uUniversidad de Zaragoza
000048112 700__ $$0(orcid)0000-0002-1075-8267$$aRemacha, Ana R.$$uUniversidad de Zaragoza
000048112 700__ $$0(orcid)0000-0002-7736-5441$$aLópez-Pérez, Óscar$$uUniversidad de Zaragoza
000048112 700__ $$aFernández-Borges, Natalia
000048112 700__ $$aCastilla, Joaquín
000048112 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, Pilar$$uUniversidad de Zaragoza
000048112 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, Juan J.$$uUniversidad de Zaragoza
000048112 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, Clementina$$uUniversidad de Zaragoza
000048112 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, Inmaculada$$uUniversidad de Zaragoza
000048112 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cSanidad Animal
000048112 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDepartamento de Anatomía, Embriología y Genética Animal$$cGenética
000048112 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDepartamento de Patología Animal$$cMedicina y Cirugía Animal
000048112 773__ $$g96 (2015), 3715-3726$$pJ. gen. virol.$$tJOURNAL OF GENERAL VIROLOGY$$x0022-1317
000048112 8564_ $$s6401384$$uhttp://zaguan.unizar.es/record/48112/files/texto_completo.pdf$$yVersión publicada
000048112 8564_ $$s106716$$uhttp://zaguan.unizar.es/record/48112/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000048112 909CO $$ooai:zaguan.unizar.es:48112$$particulos$$pdriver
000048112 951__ $$a2018-02-22-09:47:15
000048112 980__ $$aARTICLE