000048676 001__ 48676
000048676 005__ 20200221144248.0
000048676 0247_ $$2doi$$a10.1371/journal.pone.0151384
000048676 0248_ $$2sideral$$a94643
000048676 037__ $$aART-2016-94643
000048676 041__ $$aeng
000048676 100__ $$0(orcid)0000-0002-0531-0943$$aGonzález, A.$$uUniversidad de Zaragoza
000048676 245__ $$aExpanding the role of FurA as essential global regulator in cyanobacteria
000048676 260__ $$c2016
000048676 5060_ $$aAccess copy available to the general public$$fUnrestricted
000048676 5203_ $$aIn the nitrogen-fixing heterocyst-forming cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA plays a global regulatory role. Failures to eliminate wild-type copies of furA gene from the polyploid genome suggest essential functions. In the present study, we developed a selectively regulated furA expression system by the replacement of furA promoter in the Anabaena sp. chromosomes with the Co2+/Zn2+ inducible coaT promoter from Synechocystis sp. PCC 6803. By removing Co2+ and Zn2+ from the medium and shutting off furA expression, we showed that FurA was absolutely required for cyanobacterial growth. RNA-seq based comparative transcriptome analyses of the furA-turning off strain and its parental wild-type in conjunction with subsequent electrophoretic mobility shift assays and semi-quantitative RT-PCR were carried out in order to identify direct transcriptional targets and unravel new biological roles of FurA. The results of such approaches led us to identify 15 novel direct iron-dependent transcriptional targets belonging to different functional categories including detoxification and defences against oxidative stress, phycobilisome degradation, chlorophyll catabolism and programmed cell death, light sensing and response, heterocyst differentiation, exopolysaccharide biosynthesis, among others. Our analyses evidence novel interactions in the complex regulatory network orchestrated by FurA in cyanobacteria.
000048676 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B18$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458
000048676 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000048676 590__ $$a2.806$$b2016
000048676 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000048676 592__ $$a1.236$$b2016
000048676 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000048676 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000048676 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000048676 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000048676 700__ $$0(orcid)0000-0002-8181-2689$$aBes , M.T.$$uUniversidad de Zaragoza
000048676 700__ $$0(orcid)0000-0002-2742-3711$$aPeleato, M.L.$$uUniversidad de Zaragoza
000048676 700__ $$0(orcid)0000-0001-8644-4574$$aFillat, M.F.$$uUniversidad de Zaragoza
000048676 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000048676 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000048676 773__ $$g11, 3 (2016), 0151384 [22 p.]$$pPLoS One$$tPloS one$$x1932-6203
000048676 8564_ $$s1790862$$uhttps://zaguan.unizar.es/record/48676/files/texto_completo.pdf$$yVersión publicada
000048676 8564_ $$s103539$$uhttps://zaguan.unizar.es/record/48676/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000048676 909CO $$ooai:zaguan.unizar.es:48676$$particulos$$pdriver
000048676 951__ $$a2020-02-21-13:26:27
000048676 980__ $$aARTICLE