Effects of Phenolic Acids on the Growth and Production of T-2 and HT-2 Toxins by Fusarium langsethiae and F. sporotrichioides

Ferruz, E. ; Atanasova-Pénichon, V. ; Bonnin-Verdal, M. ; Marchegay, G. ; Pinson-Gadais, L. ; Ducos, C. ; Lorán, S. (Universidad de Zaragoza) ; Ariño, A. (Universidad de Zaragoza) ; Barreau, C. ; Richard-Forget, F.

Resumen: The effect of natural phenolic acids was tested on the growth and production of T-2 and HT-2 toxins by Fusarium langsethiae and F. sporotrichioides, on Mycotoxin Synthetic medium. Plates treated with 0.5 mM of each phenolic acid (caffeic, chlorogenic, ferulic and p-coumaric) and controls without phenolic acid were incubated for 14 days at 25 °C. Fungal biomass of F. langsethiae and F. sporotrichioides was not reduced by the phenolic acids. However, biosynthesis of T-2 toxin by F. langsethiae was significantly reduced by chlorogenic (23.1%) and ferulic (26.5%) acids. Production of T-2 by F. sporotrichioides also decreased with ferulic acid by 23% (p < 0.05). In contrast, p-coumaric acid significantly stimulated the production of T-2 and HT-2 toxins for both strains. A kinetic study of F. langsethiae with 1 mM ferulic acid showed a significant decrease in fungal biomass, whereas T-2 production increased after 10 days of incubation. The study of gene expression in ferulic supplemented cultures of F. langsethiae revealed a significant inhibition for Tri5, Tri6 and Tri12 genes, while for Tri16 the decrease in gene expression was not statistically significant. Overall, results indicated that phenolic acids had a variable effect on fungal growth and mycotoxin production, depending on the strain and the concentration and type of phenolic acid assayed.

Idioma: Inglés

DOI: 10.3390/molecules21040449

Año: 2016

Publicado en: Molecules 21, 4 (2016), 449 [11 pp]
ISSN: 1420-3049

Factor impacto JCR: 2.861 (2016)

Categ. JCR: CHEMISTRY, ORGANIC rank: 17 / 59 = 0.288 (2016) - Q2 - T1

Factor impacto SCIMAGO: 0.824 - Analytical Chemistry (Q1) - Pharmaceutical Science (Q1) - Chemistry (miscellaneous) (Q1) - Organic Chemistry (Q2) - Physical and Theoretical Chemistry (Q2) - Drug Discovery (Q2) - Medicine (miscellaneous) (Q2) - Molecular Medicine (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A01
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2011-26808
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2014-52648-REDT
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL 2014-57069-R

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Nutrición Bromatología (Dpto. Produc.Animal Cienc.Ali.)