000056312 001__ 56312 000056312 005__ 20210121114544.0 000056312 0248_ $$2sideral$$a91946 000056312 0247_ $$2doi$$a10.23675/sjlas.v41i0.336 000056312 037__ $$aART-2015-91946 000056312 041__ $$aeng 000056312 100__ $$0(orcid)0000-0002-6106-2577$$aMaria, GA$$uUniversidad de Zaragoza 000056312 245__ $$aSheep as an experimental model: individual housing allowing visual, auditory, olfactory and tactile contact is not an obstacle for studies involving hormonal interrelationships 000056312 260__ $$c2015 000056312 5060_ $$aAccess copy available to the general public$$fUnrestricted 000056312 5203_ $$aThirty-two ewes were used to determine whether individual housing, allowing contact with neighbours, induces a stress response. Ewes were housed in individual pens designed to allow the ewes to see, hear, smell and touch adjacent animals, and were distributed into four groups (n=8/group): ewes with subcutaneous implants containing melatonin and oestradiol (M+E), melatonin (M), oestradiol (E) and non-implanted control ewes (C). Heart rate, stress indicators (plasma cortisol, glucose, lactate and creatine kinase (CK) concentrations) and luteinizing hormone (LH) concentrations were measured hourly and compared with the resting values (before and after pen housing). Heart rate increased significantly during the introduction into the pen (P<0.001) in all groups, in comparison with the resting values. No significant differences between groups were observed for cortisol concentrations, with the exception of the M group, which showed the highest response (P<0.001) when ewes were introduced into the pens. Lactate, CK and glucose changes in comparison with the resting values were similar between groups. LH concentrations during pen housing decreased significantly in all groups in comparison with resting values. In conclusion, individual confinement of sheep allowing visual, auditory, olfactory and tactile contact with their neighbouring animals was not an obstacle for investigating particular hormonal interrelationships with multiple sampling procedures. However further investigations are required to determine if this conclusion applies to other hormone systems in sheep. 000056312 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000056312 590__ $$a0.0$$b2015 000056312 591__ $$aVETERINARY SCIENCES$$b137 / 138 = 0.993$$c2015$$dQ4$$eT3 000056312 592__ $$a0.101$$b2015 000056312 593__ $$aAnimal Science and Zoology$$c2015$$dQ4 000056312 593__ $$aVeterinary (miscellaneous)$$c2015$$dQ4 000056312 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2015$$dQ4 000056312 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000056312 700__ $$aValares, JA 000056312 700__ $$0(orcid)0000-0002-5399-8746$$aForcada, F$$uUniversidad de Zaragoza 000056312 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia Martínez, José Alfonso$$uUniversidad de Zaragoza 000056312 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000056312 773__ $$g41 (2015), [9 pp.]$$pScand. j. lab. anim. sci.$$tSCANDINAVIAN JOURNAL OF LABORATORY ANIMAL SCIENCE$$x0901-3393 000056312 85641 $$uhttp://www.sjlas.org/index.php/SJLAS/article/view/336$$zTexto completo de la revista 000056312 8564_ $$s233716$$uhttps://zaguan.unizar.es/record/56312/files/texto_completo.pdf$$yVersión publicada 000056312 8564_ $$s113207$$uhttps://zaguan.unizar.es/record/56312/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000056312 909CO $$ooai:zaguan.unizar.es:56312$$particulos$$pdriver 000056312 951__ $$a2021-01-21-11:17:57 000056312 980__ $$aARTICLE