000056748 001__ 56748
000056748 005__ 20200221144335.0
000056748 0247_ $$2doi$$a10.1371/journal.pone.0159272
000056748 0248_ $$2sideral$$a96058
000056748 037__ $$aART-2016-96058
000056748 041__ $$aeng
000056748 100__ $$aNymo, I.H.
000056748 245__ $$aExperimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata)
000056748 260__ $$c2016
000056748 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056748 5203_ $$aPathology has not been observed in true seals infected with Brucella pinnipedialis. A lack of intracellular survival and multiplication of B. pinnipedialis in hooded seal (Cystophora cristata) macrophages in vitro indicates a lack of chronic infection in hooded seals. Both epidemiology and bacteriological patterns in the hooded seal point to a transient infection of environmental origin, possibly through the food chain. To analyse the potential role of fish in the transmission of B. pinnipedialis, Atlantic cod (Gadus morhua) were injected intraperitoneally with 7.5 x 107 bacteria of a hooded seal field isolate. Samples of blood, liver, spleen, muscle, heart, head kidney, female gonads and feces were collected on days 1, 7, 14 and 28 post infection to assess the bacterial load, and to determine the expression of immune genes and the specific antibody response. Challenged fish showed an extended period of bacteremia through day 14 and viable bacteria were observed in all organs sampled, except muscle, until day 28. Neither gross lesions nor mortality were recorded. Anti-Brucella antibodies were detected from day 14 onwards and the expression of hepcidin, cathelicidin, interleukin (IL)-1ß, IL-10, and interferon (IFN)-¿ genes were significantly increased in spleen at day 1 and 28. Primary mononuclear cells isolated from head kidneys of Atlantic cod were exposed to B. pinnipedialis reference (NCTC 12890) and hooded seal (17a-1) strain. Both bacterial strains invaded mononuclear cells and survived intracellularly without any major reduction in bacterial counts for at least 48 hours. Our study shows that the B. pinnipedialis strain isolated from hooded seal survives in Atlantic cod, and suggests that Atlantic cod could play a role in the transmission of B. pinnipedialis to hooded seals in the wild.
000056748 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2013-00065-C02-01
000056748 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000056748 590__ $$a2.806$$b2016
000056748 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000056748 592__ $$a1.236$$b2016
000056748 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000056748 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000056748 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000056748 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056748 700__ $$aSeppola, M.
000056748 700__ $$aDahouk, S.A.
000056748 700__ $$aBakkemo, K.R.
000056748 700__ $$0(orcid)0000-0002-5490-5177$$aDe Bagüés, M.P.J.
000056748 700__ $$aGodfroid, J.
000056748 700__ $$aLarsen, A.K.
000056748 773__ $$g11, 7 (2016), 0159272 [18p.]$$pPLoS One$$tPloS one$$x1932-6203
000056748 8564_ $$s523244$$uhttps://zaguan.unizar.es/record/56748/files/texto_completo.pdf$$yVersión publicada
000056748 8564_ $$s108406$$uhttps://zaguan.unizar.es/record/56748/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056748 909CO $$ooai:zaguan.unizar.es:56748$$particulos$$pdriver
000056748 951__ $$a2020-02-21-13:46:46
000056748 980__ $$aARTICLE