000056831 001__ 56831
000056831 005__ 20200922122721.0
000056831 0247_ $$2doi$$a10.14306/renhyd.19.1.110
000056831 0248_ $$2sideral$$a89481
000056831 037__ $$aART-2015-89481
000056831 041__ $$aeng
000056831 100__ $$0(orcid)0000-0002-1886-9586$$aMenal-Puey Susana$$uUniversidad de Zaragoza
000056831 245__ $$aValidation of a rapid method for detecting nitrate in chard (Beta vulgaris cycla). Analysis of spanish commercial samples marketed in the region of huesca, spain, and estimation of the daily intake
000056831 260__ $$c2015
000056831 5060_ $$aAccess copy available to the general public$$fUnrestricted
000056831 5203_ $$aIntroduction: This paper presents the validation of a rapid method for the determination of nitrate content in chard samples, in order to determine, during two periods (winter and summer of 2012), the current levels of nitrate in this typical vegetable, and the toxicological risk associated with this intake.
Material and Methods: A rapid colorimetric determination of nitrate in chard samples by nitration of salicylic acid was validated. The validated method was applied to analyze the content of nitrate in 56 chard samples marketed in Huesca (Spain) and collected in winter and summer seasons, and the toxicological risk associated with the intake for adult and children population was evaluated.
Results: The method was specific and robust enough for the required purposes. The main performance characteristics of the method were: limits of detection and quantitation of 0.29 mg L-1 and 0.59 mg L-1, respectively; recoveries from 80.0% to 107.4%; and coefficients of variation lower than 11.4%. The detected mean nitrate content was 2293 mg kg-1 and there was evidence of risk only for extreme consumers (adults and children), especially in winter period.
Conclusions: A high percentage of chard samples with a considerable concentration of nitrate were found. Taking into account the estimated dairy intake of nitrate associated with them, it could be recommended to establish a regulatory limit of nitrate to chard, a vegetable of important consumption in Spain.
000056831 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T10$$9info:eu-repo/grantAgreement/ES/DGA/A01
000056831 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-sa$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/es/
000056831 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000056831 700__ $$0(orcid)0000-0001-8822-7303$$aAsensio Casas, Esther$$uUniversidad de Zaragoza
000056831 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000056831 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000056831 773__ $$g19, 1 (2015), 4-11$$pRev. esp. nutr. hum. diet.$$tRevista española de nutrición humana y dietética$$x2173-1292
000056831 8564_ $$s957935$$uhttp://zaguan.unizar.es/record/56831/files/texto_completo.pdf$$yVersión publicada
000056831 8564_ $$s81503$$uhttp://zaguan.unizar.es/record/56831/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000056831 909CO $$ooai:zaguan.unizar.es:56831$$particulos$$pdriver
000056831 951__ $$a2020-09-22-12:18:51
000056831 980__ $$aARTICLE