000057765 001__ 57765
000057765 005__ 20200221144342.0
000057765 0247_ $$2doi$$a10.4103/1008-682X.187579
000057765 0248_ $$2sideral$$a97125
000057765 037__ $$aART-2016-97125
000057765 041__ $$aeng
000057765 100__ $$aValverde, A.
000057765 245__ $$aMorphometry and subpopulation structure of Holstein bull spermatozoa: Variations in ejaculates and cryopreservation straws
000057765 260__ $$c2016
000057765 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057765 5203_ $$aSperm quality is evaluated for the calculation of sperm dosage in artificial reproductive programs. The most common parameter used is motility, but morphology has a higher potential as a predictor of genetic quality. Morphometry calculations from CASA-Morph technology improve morphological evaluation and allow mathematical approaches to the problem. Semen from 28 Holstein bulls was collected by artificial vagina, and several ejaculates were studied. After general evaluation, samples were diluted, packaged in 0.25 ml straws, and stored in liquid nitrogen. Two straws per sample were thawed, and slides were processed and stained with Diff-Quik. Samples were analyzed by a CASA-Morph system for eight morphometric parameters. In addition to the «classical» statistical approach, based on variance analysis (revealing differences between animals, ejaculates, and straws), principal component (PC) analysis showed that the variables were grouped into PC1, related to size, and PC2 to shape. Subpopulation structure analysis showed four groups, namely, big, small, short, and narrow from their dominant characteristics, representing 31.0%, 27.3%, 24.1%, and 17.7% of the total population, respectively. The distributions varied between animals and ejaculates, but between straws, there were no differences in only four animals. This modern approach of considering an ejaculate sperm population as divided into subpopulations reflecting quantifiable parameters generated by CASA-Morph systems technology opens a new view on sperm function. This is the first study applying this approach to evaluate different ejaculates and straws from the same individual. More work must be done to improve seminal dose calculations in assisted reproductive programs.
000057765 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057765 590__ $$a2.996$$b2016
000057765 591__ $$aANDROLOGY$$b1 / 5 = 0.2$$c2016$$dQ1$$eT1
000057765 591__ $$aUROLOGY & NEPHROLOGY$$b22 / 76 = 0.289$$c2016$$dQ2$$eT1
000057765 592__ $$a0.793$$b2016
000057765 593__ $$aUrology$$c2016$$dQ2
000057765 593__ $$aMedicine (miscellaneous)$$c2016$$dQ2
000057765 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057765 700__ $$aArenán, H.
000057765 700__ $$aSancho, M.
000057765 700__ $$aContell, J.
000057765 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.$$uUniversidad de Zaragoza
000057765 700__ $$aFernández, A.
000057765 700__ $$aSoler, C.
000057765 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000057765 773__ $$g18, 6 (2016), 851-857$$pAsian J. Androl.$$tAsian journal of andrology$$x1008-682X
000057765 8564_ $$s688707$$uhttps://zaguan.unizar.es/record/57765/files/texto_completo.pdf$$yVersión publicada
000057765 8564_ $$s128194$$uhttps://zaguan.unizar.es/record/57765/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057765 909CO $$ooai:zaguan.unizar.es:57765$$particulos$$pdriver
000057765 951__ $$a2020-02-21-13:50:52
000057765 980__ $$aARTICLE