000057797 001__ 57797
000057797 005__ 20200221144348.0
000057797 0247_ $$2doi$$a10.4103/1008-682X.187578
000057797 0248_ $$2sideral$$a97111
000057797 037__ $$aART-2016-97111
000057797 041__ $$aeng
000057797 100__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza
000057797 245__ $$aComputer-assisted sperm morphometry fluorescence-based analysis has potential to determine progeny sex
000057797 260__ $$c2016
000057797 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057797 5203_ $$aThis study was designed to determine the ability of computer-assisted sperm morphometry analysis (CASA-Morph) with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa (SX and SY, respectively). Spermatozoa from eight bulls were processed to assess simultaneously the sex chromosome by FISH and sperm morphometry by fluorescence-based CASA-Morph. SX cells were larger than SY cells on average (P < 0.001) although with important differences between bulls. A simultaneous evaluation of all the measured features by discriminant analysis revealed that nuclear area and average fluorescence intensity were the variables selected by stepwise discriminant function analysis as the best discriminators between SX and SY. In the second experiment, the sperm nuclear morphometric results from CASA-Morph in nonsexed (mixed SX and SY) and sexed (SX) semen samples from four bulls were compared. FISH allowed a successful classification of spermatozoa according to their sex chromosome content. X-sexed spermatozoa displayed a larger size and fluorescence intensity than nonsexed spermatozoa (P < 0.05). We conclude that the CASA-Morph fluorescence-based method has the potential to find differences between X- and Y-chromosome-bearing spermatozoa in bovine species although more studies are needed to increase the precision of sex determination by this technique.
000057797 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A40$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
000057797 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057797 590__ $$a2.996$$b2016
000057797 591__ $$aANDROLOGY$$b1 / 5 = 0.2$$c2016$$dQ1$$eT1
000057797 591__ $$aUROLOGY & NEPHROLOGY$$b22 / 76 = 0.289$$c2016$$dQ2$$eT1
000057797 592__ $$a0.793$$b2016
000057797 593__ $$aUrology$$c2016$$dQ2
000057797 593__ $$aMedicine (miscellaneous)$$c2016$$dQ2
000057797 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057797 700__ $$aPauciullo, A.
000057797 700__ $$aSilvestre, M.
000057797 700__ $$aVicente-Fiel, S.
000057797 700__ $$aVillanova, L.
000057797 700__ $$aPinton, A.
000057797 700__ $$0(orcid)0000-0001-5658-8411$$aViruel, J.
000057797 700__ $$0(orcid)0000-0003-0582-5418$$aSales, E.$$uUniversidad de Zaragoza
000057797 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.$$uUniversidad de Zaragoza
000057797 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000057797 7102_ $$15011$$2705$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Producción Vegetal
000057797 773__ $$g18, 6 (2016), 858-862$$pAsian J. Androl.$$tAsian journal of andrology$$x1008-682X
000057797 8564_ $$s324137$$uhttps://zaguan.unizar.es/record/57797/files/texto_completo.pdf$$yVersión publicada
000057797 8564_ $$s126056$$uhttps://zaguan.unizar.es/record/57797/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057797 909CO $$ooai:zaguan.unizar.es:57797$$particulos$$pdriver
000057797 951__ $$a2020-02-21-13:53:44
000057797 980__ $$aARTICLE