000057798 001__ 57798
000057798 005__ 20200221144337.0
000057798 0247_ $$2doi$$a10.4103/1008-682X.186874
000057798 0248_ $$2sideral$$a97112
000057798 037__ $$aART-2016-97112
000057798 041__ $$aeng
000057798 100__ $$0(orcid)0000-0001-8991-325X$$aSantolaria, P.$$uUniversidad de Zaragoza
000057798 245__ $$aMorphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men
000057798 260__ $$c2016
000057798 5060_ $$aAccess copy available to the general public$$fUnrestricted
000057798 5203_ $$aThis study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kinematic subpopulations (slow with low ALH 35.6% of all motile spermatozoa], with circular trajectories 32.0%], and rapid with high ALH 32.4%]), and three morphometric subpopulations (large-round 33.9% of all spermatozoa], elongated 32.0%], and small 34.10%]). The distribution of kinematic sperm subpopulations was different among ejaculate fractions (P < 0.001), with higher percentages of spermatozoa exhibiting slow movements with low ALH in the second and third portions, and with a more homogeneous distribution of kinematic sperm subpopulations in the first portion. The distribution of morphometric sperm subpopulations was also different among ejaculate fractions (P < 0.001), with more elongated spermatozoa in the first, and of small spermatozoa in the third, portion. It is concluded that important variations in the distribution of kinematic and morphometric sperm subpopulations exist between ejaculate fractions, with possible functional implications.
000057798 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A40$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P
000057798 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000057798 590__ $$a2.996$$b2016
000057798 591__ $$aANDROLOGY$$b1 / 5 = 0.2$$c2016$$dQ1$$eT1
000057798 591__ $$aUROLOGY & NEPHROLOGY$$b22 / 76 = 0.289$$c2016$$dQ2$$eT1
000057798 592__ $$a0.793$$b2016
000057798 593__ $$aUrology$$c2016$$dQ2
000057798 593__ $$aMedicine (miscellaneous)$$c2016$$dQ2
000057798 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000057798 700__ $$aSoler, C.
000057798 700__ $$0(orcid)0000-0001-5359-6037$$aRecreo, P.$$uUniversidad de Zaragoza
000057798 700__ $$0(orcid)0000-0001-6202-3089$$aCarretero, T.$$uUniversidad de Zaragoza
000057798 700__ $$aBono, A.
000057798 700__ $$aBerné, J.
000057798 700__ $$0(orcid)0000-0001-5316-1703$$aYániz, J.$$uUniversidad de Zaragoza
000057798 7102_ $$11003$$2027$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Anatom.Embriol.Humana
000057798 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000057798 773__ $$g18, 6 (2016), 831-834$$pAsian J. Androl.$$tAsian journal of andrology$$x1008-682X
000057798 8564_ $$s252340$$uhttps://zaguan.unizar.es/record/57798/files/texto_completo.pdf$$yVersión publicada
000057798 8564_ $$s128653$$uhttps://zaguan.unizar.es/record/57798/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000057798 909CO $$ooai:zaguan.unizar.es:57798$$particulos$$pdriver
000057798 951__ $$a2020-02-21-13:48:17
000057798 980__ $$aARTICLE