000058439 001__ 58439 000058439 005__ 20210202111953.0 000058439 0247_ $$2doi$$a10.3389/fgene.2016.00198 000058439 0248_ $$2sideral$$a97423 000058439 037__ $$aART-2016-97423 000058439 041__ $$aeng 000058439 100__ $$aBadenes, M.L. 000058439 245__ $$aApplication of genomic technologies to the breeding of trees 000058439 260__ $$c2016 000058439 5060_ $$aAccess copy available to the general public$$fUnrestricted 000058439 5203_ $$aThe recent introduction of next generation sequencing (NGS) technologies represents a major revolution in providing new tools for identifying the genes and/or genomic intervals controlling important traits for selection in breeding programs. In perennial fruit trees with long generation times and large sizes of adult plants, the impact of these techniques is even more important. High-throughput DNA sequencing technologies have provided complete annotated sequences in many important tree species. Most of the high-throughput genotyping platforms described are being used for studies of genetic diversity and population structure. Dissection of complex traits became possible through the availability of genome sequences along with phenotypic variation data, which allow to elucidate the causative genetic differences that give rise to observed phenotypic variation. Association mapping facilitates the association between genetic markers and phenotype in unstructured and complex populations, identifying molecular markers for assisted selection and breeding. Also, genomic data provide in silico identification and characterization of genes and gene families related to important traits, enabling new tools for molecular marker assisted selection in tree breeding. Deep sequencing of transcriptomes is also a powerful tool for the analysis of precise expression levels of each gene in a sample. It consists in quantifying short cDNA reads, obtained by NGS technologies, in order to compare the entire transcriptomes between genotypes and environmental conditions. The miRNAs are non-coding short RNAs involved in the regulation of different physiological processes, which can be identified by high-throughput sequencing of RNA libraries obtained by reverse transcription of purified short RNAs, and by in silico comparison with known miRNAs from other species. All together, NGS techniques and their applications have increased the resources for plant breeding in tree species, closing the former gap of genetic tools between trees and annual species. 000058439 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2013-00026$$9info:eu-repo/grantAgreement/ES/INIA/RTA2014-00062 000058439 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000058439 590__ $$a3.789$$b2016 000058439 591__ $$aGENETICS & HEREDITY$$b47 / 166 = 0.283$$c2016$$dQ2$$eT1 000058439 592__ $$a2.067$$b2016 000058439 593__ $$aGenetics$$c2016$$dQ1 000058439 593__ $$aMolecular Medicine$$c2016$$dQ1 000058439 593__ $$aGenetics (clinical)$$c2016$$dQ1 000058439 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000058439 700__ $$aFernández i Marti, A. 000058439 700__ $$aRíos, G. 000058439 700__ $$0(orcid)0000-0002-3455-0145$$aRubio-Cabetas, M.J. 000058439 773__ $$g7 (2016), 198 [13 pp.]$$pFront. genet.$$tFrontiers in Genetics$$x1664-8021 000058439 8564_ $$s355271$$uhttps://zaguan.unizar.es/record/58439/files/texto_completo.pdf$$yVersión publicada 000058439 8564_ $$s20223$$uhttps://zaguan.unizar.es/record/58439/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000058439 909CO $$ooai:zaguan.unizar.es:58439$$particulos$$pdriver 000058439 951__ $$a2021-02-02-11:13:48 000058439 980__ $$aARTICLE