000058462 001__ 58462
000058462 005__ 20200221144327.0
000058462 0247_ $$2doi$$a10.3389/fpls.2016.01715
000058462 0248_ $$2sideral$$a97508
000058462 037__ $$aART-2016-97508
000058462 041__ $$aeng
000058462 100__ $$aKsouri, N.
000058462 245__ $$aTranscriptional responses in root and leaf of prunus persica under drought stress using RNA sequencing
000058462 260__ $$c2016
000058462 5060_ $$aAccess copy available to the general public$$fUnrestricted
000058462 5203_ $$aPrunus persica L. Batsch, or peach, is one of the most important crops and it is widely established in irrigated arid and semi-arid regions. However, due to variations in the climate and the increased aridity, drought has become a major constraint, causing crop losses worldwide. The use of drought-tolerant rootstocks in modern fruit production appears to be a useful method of alleviating water deficit problems. However, the transcriptomic variation and the major molecular mechanisms that underlie the adaptation of drought-tolerant rootstocks to water shortage remain unclear. Hence, in this study, high-throughput sequencing (RNA-seq) was performed to assess the transcriptomic changes and the key genes involved in the response to drought in root tissues (GF677 rootstock) and leaf tissues (graft, var. Catherina) subjected to 16 days of drought stress. In total, 12 RNA libraries were constructed and sequenced. This generated a total of 315 M raw reads from both tissues, which allowed the assembly of 22, 079 and 17, 854 genes associated with the root and leaf tissues, respectively. Subsets of 500 differentially expressed genes (DEGs) in roots and 236 in leaves were identified and functionally annotated with 56 gene ontology (GO) terms and 99 metabolic pathways, which were mostly associated with aminobenzoate degradation and phenylpropanoid biosynthesis. The GO analysis highlighted the biological functions that were exclusive to the root tissue, such as “locomotion, ” “hormone metabolic process, ” and “detection of stimulus, ” indicating the stress-buffering role of the GF677 rootstock. Furthermore, the complex regulatory network involved in the drought response was revealed, involving proteins that are associated with signaling transduction, transcription and hormone regulation, redox homeostasis, and frontline barriers. We identified two poorly characterized genes in P. persica: growth-regulating factor 5 (GRF5), which may be involved in cellular expansion, and AtHB12, which may be involved in root elongation. The reliability of the RNA-seq experiment was validated by analyzing the expression patterns of 34 DEGs potentially involved in drought tolerance using quantitative reverse transcription polymerase chain reaction. The transcriptomic resources generated in this study provide a broad characterization of the acclimation of P. persica to drought, shedding light on the major molecular responses to the most important environmental stressor.
000058462 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000058462 590__ $$a4.298$$b2016
000058462 591__ $$aPLANT SCIENCES$$b20 / 211 = 0.095$$c2016$$dQ1$$eT1
000058462 592__ $$a1.973$$b2016
000058462 593__ $$aPlant Science$$c2016$$dQ1
000058462 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000058462 700__ $$aJiménez, S.
000058462 700__ $$aWells, C.E.
000058462 700__ $$0(orcid)0000-0002-5462-907X$$aContreras-Moreira, B.$$uUniversidad de Zaragoza
000058462 700__ $$0(orcid)0000-0003-1081-430X$$aGogorcena, Y.
000058462 7102_ $$15011$$2063$$aUniversidad de Zaragoza$$bDpto. CC.Agrar.y Medio Natural$$cÁrea Botánica
000058462 773__ $$g7 (2016), 1715 [19 pp]$$pFront. plant sci.$$tFRONTIERS IN PLANT SCIENCE$$x1664-462X
000058462 8564_ $$s821114$$uhttps://zaguan.unizar.es/record/58462/files/texto_completo.pdf$$yVersión publicada
000058462 8564_ $$s24152$$uhttps://zaguan.unizar.es/record/58462/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000058462 909CO $$ooai:zaguan.unizar.es:58462$$particulos$$pdriver
000058462 951__ $$a2020-02-21-13:44:47
000058462 980__ $$aARTICLE