000058647 001__ 58647
000058647 005__ 20170831220643.0
000058647 037__ $$aTAZ-TFM-2016-822
000058647 041__ $$aspa
000058647 1001_ $$aMora Sanz, Verónica
000058647 24200 $$aFirst steps towards the design of an enzymatic optical (nano)biosensor for the determination of glucose.
000058647 24500 $$aFirst steps towards the design of an enzymatic optical (nano)biosensor for the determination of glucose.
000058647 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2016
000058647 500__ $$aAporta en secretaría material físico.
000058647 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000058647 520__ $$aThis study describes the first steps towards the design of an enzymatic optical (nano)biosensor for the determination of glucose. The aim of this work is to relate the optical properties of gold nanomaterials with glucose concentration. In order to achieve this goal, the redox properties of FAD cofactor of the glucose oxidase enzyme were used. During the enzymatic reaction between glucose oxidase and glucose, the FAD cofactor is reduced to FADH2. A gold precursor in oxidation state 3+ is used to reoxidize the enzyme which at the same time is reduced to gold in oxidation state 0. Gold in oxidation state 0 can be developed into two different types of nanomaterials: gold nanoparticles (AuNPs) or gold nanoclusters (AuNCs). These nanomaterials have different optical properties which could be related to the glucose concentration. On the one hand AuNPs exhibit absorption properties due to surface plasmon resonance (SPR). On the other hand AuNCs are composed by only few atoms, its size is around 2 nm or less and displays fluorescence. The intensity of SPR of gold nanoparticles has been successfully related to the glucose concentration. In addition, kinetics studies have shown a relationship between the velocity at which the fluorescent intensity of AuNCs increases and the glucose concentration.
000058647 521__ $$aMáster Universitario en Materiales Nanoestructurados para Aplicaciones Nanotecnológicas (Nanostructured Materials for Nanotechnology Applications)
000058647 540__ $$aDerechos regulados por licencia Creative Commons
000058647 700__ $$ade Marcos Ruiz, Susana$$edir.
000058647 700__ $$aGalbán Bernal, Francisco Javier$$edir.
000058647 7102_ $$aUniversidad de Zaragoza$$bQuímica Analítica$$cQuímica Analítica
000058647 8560_ $$f646073@celes.unizar.es
000058647 8564_ $$s2280756$$uhttps://zaguan.unizar.es/record/58647/files/TAZ-TFM-2016-822.pdf$$yMemoria (spa)
000058647 909CO $$ooai:zaguan.unizar.es:58647$$pdriver$$ptrabajos-fin-master
000058647 950__ $$a
000058647 951__ $$adeposita:2017-01-26
000058647 980__ $$aTAZ$$bTFM$$cCIEN