A Hyphenated Technique based on High-Performance Thin Layer Chromatography for Determining Neutral Sphingolipids: A Proof of concept
Resumen: Hyphenated HPTLC has been used to analyze several neutral sphingolipids acting as lysosomal storage disease (LSD) biomarkers. Automated multiple development (AMD) provides separation of lipid peaks, which are detected and quantified using fluorescence detection by intensity changes (FDIC) after primuline post-impregnation. A final online transfer to a mass spectrometer by means of an elution-based interface allows their identification using electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI).Given that the increases in fluorescent emission detected by FDIC are produced by non-specific, electrostatic interactions between the primuline and hydrocarbon chains in the ceramide backbones of sphingolipids, it is a non-destructive detection technique, allowing the precise location and transfer of biomarker peaks to a mass spectrometer using an elution interface. By using primuline as a fluorophore, the technique is also compatible with ESI-APCI and does not interfere with the MS of sphingolipids. APCI provides useful and complementary structural information to the ESI for sphingolipid identification. Moreover, FDIC emission can be used for quantitative purposes. Results include the determination of sphingomyelin (SM) in human-plasma samples (RSD < 6%) by means of a standard addition method with non-linear calibration, and the identification of globotriaosylceramide (Gb3) in the plasma of a Fabry patient. Only one HPTLC plate is needed to perform the analysis.
Idioma: Inglés
DOI: 10.3390/chromatography2020167
Año: 2015
Publicado en: Chromatography (Basel) 2, 2 (2015), 167-187
ISSN: 2227-9075

Factor impacto SCIMAGO:

Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-035535
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2012-34774
Tipo y forma: Article (Published version)
Área (Departamento): Química Física (Departamento de Química Física)
Área (Departamento): Química Orgánica (Departamento de Química Orgánica)
Área (Departamento): Química Analítica (Departamento de Química Analítica)


Creative Commons You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.


Exportado de SIDERAL (2017-03-13-13:11:55)

Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Química Analítica
Articles > Artículos por área > Química Orgánica
Articles > Artículos por área > Química Física



 Record created 2017-03-13, last modified 2017-03-27


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