000060888 001__ 60888 000060888 005__ 20180222094945.0 000060888 0247_ $$2doi$$a10.1128/JCM.02519-13 000060888 0248_ $$2sideral$$a85173 000060888 037__ $$aART-2014-85173 000060888 041__ $$aeng 000060888 100__ $$aAllix-Béguec, C. 000060888 245__ $$aProposal of a consensus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem-repeat loci for subtyping of mycobacterium tuberculosis Beijing isolates 000060888 260__ $$c2014 000060888 5060_ $$aAccess copy available to the general public$$fUnrestricted 000060888 5203_ $$aMycobacterium tuberculosis Beijing strains represent targets of special importance for molecular surveillance of tuberculosis (TB), especially because they are associated with spread of multidrug resistance in some world regions. Standard 24-locus mycobacterial interspersed repetitive-unit–variable-number tandem-repeat (MIRU-VNTR) typing lacks resolution power for accurately discriminating closely related clones that often compose Beijing strain populations. Therefore, we evaluated a set of 7 additional, hypervariable MIRU-VNTR loci for better resolution and tracing of such strains, using a collection of 535 Beijing isolates from six world regions where these strains are known to be prevalent. The typeability and interlaboratory reproducibility of these hypervariable loci were lower than those of the 24 standard loci. Three loci (2163a, 3155, and 3336) were excluded because of their redundant variability and/or more frequent noninterpretable results compared to the 4 other markers. The use of the remaining 4-locus set (1982, 3232, 3820, and 4120) increased the number of types by 52% (from 223 to 340) and reduced the clustering rate from 58.3 to 36.6%, when combined with the use of the standard 24-locus set. Known major clonal complexes/24-locus-based clusters were all subdivided, although the degree of subdivision varied depending on the complex. Only five single-locus variations were detected among the hypervariable loci of an additional panel of 92 isolates, representing 15 years of clonal spread of a single Beijing strain in a geographically restricted setting. On this calibrated basis, we propose this 4-locus set as a consensus for subtyping Beijing clonal complexes and clusters, after standard typing. 000060888 536__ $$9info:eu-repo/grantAgreement/EUR/ERDF/Oseo-A0906016N-FS 000060888 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/ 000060888 590__ $$a3.993$$b2014 000060888 591__ $$aMICROBIOLOGY$$b26 / 119 = 0.218$$c2014$$dQ1$$eT1 000060888 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000060888 700__ $$aWahl, C. 000060888 700__ $$aHanekom, M. 000060888 700__ $$aNikolayevskyy, V. 000060888 700__ $$aDrobniewski, F. 000060888 700__ $$aMaeda, S. 000060888 700__ $$aCampos-Herrero, I. 000060888 700__ $$aMokrousov, I. 000060888 700__ $$aNiemann, S. 000060888 700__ $$aKontsevaya, I. 000060888 700__ $$aRastogi, N. 000060888 700__ $$0(orcid)0000-0002-3011-2519$$aSamper, S. 000060888 700__ $$aSng, L.H. 000060888 700__ $$aWarren, R.M. 000060888 700__ $$aSupply, P. 000060888 773__ $$g52, 1 (2014), 164-172$$pJ. clin. microbiol.$$tJOURNAL OF CLINICAL MICROBIOLOGY$$x0095-1137 000060888 8564_ $$s1212148$$uhttps://zaguan.unizar.es/record/60888/files/texto_completo.pdf$$yVersión publicada 000060888 8564_ $$s135482$$uhttps://zaguan.unizar.es/record/60888/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000060888 909CO $$ooai:zaguan.unizar.es:60888$$particulos$$pdriver 000060888 951__ $$a2018-02-22-09:47:43 000060888 980__ $$aARTICLE