Global study of is6110 in a successful mycobacterium tuberculosis strain: Clues for deciphering its behavior and for its rapid detection

Millan-Lou, M.I. ; López-Calleja, A.I. ; Colmenarejo, C. ; Lezcano, M.A. ; Vitoria, M.A. (Universidad de Zaragoza) ; Del Portillo, P. ; Otal, I. (Universidad de Zaragoza) ; Martín, C. ; Samper, S.

Resumen: The Mycobacterium tuberculosis insertion sequence IS6110, besides being a very useful tool in molecular epidemiology, seems to have an impact on the biology of bacilli. In the present work, we mapped the 12 points of insertion of IS6110 in the genome of a successful strain named M. tuberculosis Zaragoza (which has been referred to as the MTZ strain). This strain, belonging to principal genetic group 3, caused a large unsuspected tuberculosis outbreak involving 85 patients in Zaragoza, Spain, in 2001 to 2004. The mapping of the points of insertion of IS6110 in the genome of the Zaragoza strain offers clues for a better understanding of the adaptability and virulence of M. tuberculosis. Surprisingly, the presence of one copy of IS6110 was found in Rv2286c, as was recently described for a successful Beijing sublineage. As a result of this analysis, a rapid method for detecting this particular M. tuberculosis strain has been designed.

Idioma: Inglés

DOI: 10.1128/JCM.00970-13

Año: 2013

Publicado en: JOURNAL OF CLINICAL MICROBIOLOGY 51, 11 (2013), 3631-3637
ISSN: 0095-1137

Factor impacto JCR: 4.232 (2013)

Categ. JCR: MICROBIOLOGY rank: 23 / 118 = 0.195 (2013) - Q1 - T1

Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS09-051
Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS12/1970
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CM09-000123

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Microbiología (Departamento de Microbiología, Medicina Preventiva y Salud Pública)