000061335 001__ 61335
000061335 005__ 20211117102002.0
000061335 0247_ $$2doi$$a10.1186/1471-2164-14-422
000061335 0248_ $$2sideral$$a95126
000061335 037__ $$aART-2013-95126
000061335 041__ $$aeng
000061335 100__ $$0(orcid)0000-0003-2742-8827$$aHenar, Alonso$$uUniversidad de Zaragoza
000061335 245__ $$aMapping IS6110 in high-copy number Mycobacterium tuberculosis strains shows specific insertion points in the Beijing genotype
000061335 260__ $$c2013
000061335 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061335 5203_ $$aBackground: Mycobacterium tuberculosis Beijing strains are characterized by a large number of IS6110 copies, suggesting the potential implication of this element in the virulence and capacity for rapid dissemination characteristic of this family. This work studies the insetion points of IS6110 in high-copy clinical isolates specifically focusing on the Beijing genotype.
Results: In the present work we mapped the insertion points of IS6110 in all the Beijing strains available in the literature and in the DNA sequence databases. We generated a representative primer collection of the IS6110 locations, which was used to analyse 61 high-copy clinical isolates. A total of 440 points of insertion were identified and analysis of their flanking regions determined the exact location, the direct repeats (DRs), the orientation and the distance to neighboring genes of each copy of IS6110. We identified specific points of insertion in Beijing strains that enabled us to obtain a dendrogram that groups the Beijing genotype. 
Conclusions: This work presents a detailed analysis of locations of IS6110 in high-copy clinical isolates, showing points of insertion present with high frequency in the Beijing family and absent in other strains.
Keywords: IS6110, Insertion points, M. tuberculosis, Beijing genotype.
000061335 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BIO2011-23555$$9info:eu-repo/grantAgreement/EC/FP7/200973/EU/Mycobacterium tuberculosis W-Beijing genetic diversity and differential virulence and host immune responses/TB-VIR$$9info:eu-repo/grantAgreement/ES/FIS/FIS09-051$$9info:eu-repo/grantAgreement/ES/DGA/B034-09
000061335 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061335 590__ $$a4.041$$b2013
000061335 591__ $$aGENETICS & HEREDITY$$b40 / 165 = 0.242$$c2013$$dQ1$$eT1
000061335 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b29 / 165 = 0.176$$c2013$$dQ1$$eT1
000061335 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061335 700__ $$0(orcid)0000-0002-3011-2519$$aSamper, Sofía
000061335 700__ $$0(orcid)0000-0003-2993-5478$$aMartín, Carlos$$uUniversidad de Zaragoza
000061335 700__ $$0(orcid)0000-0003-1027-7583$$aOtal Gil, María Isabel$$uUniversidad de Zaragoza
000061335 7102_ $$11008$$2630$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Microbiología
000061335 773__ $$g14, 422 (2013), [11 pp.]$$pBMC genomics$$tBMC Genomics$$x1471-2164
000061335 8564_ $$s1569531$$uhttps://zaguan.unizar.es/record/61335/files/texto_completo.pdf$$yVersión publicada
000061335 8564_ $$s109128$$uhttps://zaguan.unizar.es/record/61335/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061335 909CO $$ooai:zaguan.unizar.es:61335$$particulos$$pdriver
000061335 951__ $$a2021-11-15-14:34:01
000061335 980__ $$aARTICLE