Mapping IS6110 in high-copy number Mycobacterium tuberculosis strains shows specific insertion points in the Beijing genotype
Financiación FP7 / Fp7 Funds
Resumen: Background: Mycobacterium tuberculosis Beijing strains are characterized by a large number of IS6110 copies, suggesting the potential implication of this element in the virulence and capacity for rapid dissemination characteristic of this family. This work studies the insetion points of IS6110 in high-copy clinical isolates specifically focusing on the Beijing genotype.
Results: In the present work we mapped the insertion points of IS6110 in all the Beijing strains available in the literature and in the DNA sequence databases. We generated a representative primer collection of the IS6110 locations, which was used to analyse 61 high-copy clinical isolates. A total of 440 points of insertion were identified and analysis of their flanking regions determined the exact location, the direct repeats (DRs), the orientation and the distance to neighboring genes of each copy of IS6110. We identified specific points of insertion in Beijing strains that enabled us to obtain a dendrogram that groups the Beijing genotype.
Conclusions: This work presents a detailed analysis of locations of IS6110 in high-copy clinical isolates, showing points of insertion present with high frequency in the Beijing family and absent in other strains.
Keywords: IS6110, Insertion points, M. tuberculosis, Beijing genotype.

Idioma: Inglés
DOI: 10.1186/1471-2164-14-422
Año: 2013
Publicado en: BMC Genomics 14, 422 (2013), [11 pp.]
ISSN: 1471-2164

Factor impacto JCR: 4.041 (2013)
Categ. JCR: GENETICS & HEREDITY rank: 40 / 165 = 0.242 (2013) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 29 / 165 = 0.176 (2013) - Q1 - T1

Financiación: info:eu-repo/grantAgreement/ES/DGA/B034-09
Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS09-051
Financiación: info:eu-repo/grantAgreement/EC/FP7/200973/EU/Mycobacterium tuberculosis W-Beijing genetic diversity and differential virulence and host immune responses/TB-VIR
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2011-23555
Tipo y forma: Article (Published version)
Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)

Creative Commons You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.


Exportado de SIDERAL (2021-11-15-14:34:01)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Articles



 Record created 2017-05-19, last modified 2021-11-17


Versión publicada:
 PDF
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)