Transcriptomic profiling of TK2 deficient human skeletal muscle suggests a role for the p53 signalling pathway and identifies growth and differentiation factor-15 as a potential novel biomarker for mitochondrial myopathies
Kalko, S.G. ; Paco, S. ; Jou, C. ; Rodríguez, M.A. ; Meznaric, M. ; Rogac, M. ; Jekovec-Vrhovsek, M. ; Sciacco, M. ; Moggio, M. ; Fagiolari, G. ; De Paepe, B. ; De Meirleir, L. ; Ferrer, I. ; Roig-Quilis, M. ; Munell, F. ; Montoya, J. (Universidad de Zaragoza) ; López-Gallardo, E. ; Ruiz-Pesini, E. (Universidad de Zaragoza) ; Artuch, R. ; Montero, R. ; Torner, F. ; Nascimento, A. ; Ortez, C. ; Colomer, J. ; Jimenez-Mallebrera, C.
Resumen: Background
Mutations in the gene encoding thymidine kinase 2 (TK2) result in the myopathic form of mitochondrial DNA depletion syndrome which is a mitochondrial encephalomyopathy presenting in children. In order to unveil some of the mechanisms involved in this pathology and to identify potential biomarkers and therapeutic targets we have investigated the gene expression profile of human skeletal muscle deficient for TK2 using cDNA microarrays.
Results
We have analysed the whole transcriptome of skeletal muscle from patients with TK2 mutations and compared it to normal muscle and to muscle from patients with other mitochondrial myopathies. We have identified a set of over 700 genes which are differentially expressed in TK2 deficient muscle. Bioinformatics analysis reveals important changes in muscle metabolism, in particular, in glucose and glycogen utilisation, and activation of the starvation response which affects aminoacid and lipid metabolism. We have identified those transcriptional regulators which are likely to be responsible for the observed changes in gene expression.
Conclusion
Our data point towards the tumor suppressor p53 as the regulator at the centre of a network of genes which are responsible for a coordinated response to TK2 mutations which involves inflammation, activation of muscle cell death by apoptosis and induction of growth and differentiation factor 15 (GDF-15) in muscle and serum. We propose that GDF-15 may represent a potential novel biomarker for mitochondrial dysfunction although further studies are required.
Idioma: Inglés
DOI: 10.1186/1471-2164-15-91
Año: 2014
Publicado en: BMC Genomics 15, 1 (2014), 91 [22 PP]
ISSN: 1471-2164
Factor impacto JCR: 3.986 (2014)
Categ. JCR: GENETICS & HEREDITY rank: 39 / 166 = 0.235 (2014) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 26 / 163 = 0.16 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA/B33
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI10-00662
Financiación: info:eu-repo/grantAgreement/ES/MICINN/ISCIII-CP09-00011
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Exportado de SIDERAL (2017-06-12-09:14:23)
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Mutations in the gene encoding thymidine kinase 2 (TK2) result in the myopathic form of mitochondrial DNA depletion syndrome which is a mitochondrial encephalomyopathy presenting in children. In order to unveil some of the mechanisms involved in this pathology and to identify potential biomarkers and therapeutic targets we have investigated the gene expression profile of human skeletal muscle deficient for TK2 using cDNA microarrays.
Results
We have analysed the whole transcriptome of skeletal muscle from patients with TK2 mutations and compared it to normal muscle and to muscle from patients with other mitochondrial myopathies. We have identified a set of over 700 genes which are differentially expressed in TK2 deficient muscle. Bioinformatics analysis reveals important changes in muscle metabolism, in particular, in glucose and glycogen utilisation, and activation of the starvation response which affects aminoacid and lipid metabolism. We have identified those transcriptional regulators which are likely to be responsible for the observed changes in gene expression.
Conclusion
Our data point towards the tumor suppressor p53 as the regulator at the centre of a network of genes which are responsible for a coordinated response to TK2 mutations which involves inflammation, activation of muscle cell death by apoptosis and induction of growth and differentiation factor 15 (GDF-15) in muscle and serum. We propose that GDF-15 may represent a potential novel biomarker for mitochondrial dysfunction although further studies are required.
Idioma: Inglés
DOI: 10.1186/1471-2164-15-91
Año: 2014
Publicado en: BMC Genomics 15, 1 (2014), 91 [22 PP]
ISSN: 1471-2164
Factor impacto JCR: 3.986 (2014)
Categ. JCR: GENETICS & HEREDITY rank: 39 / 166 = 0.235 (2014) - Q1 - T1
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 26 / 163 = 0.16 (2014) - Q1 - T1
Financiación: info:eu-repo/grantAgreement/ES/DGA/B33
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI10-00662
Financiación: info:eu-repo/grantAgreement/ES/MICINN/ISCIII-CP09-00011
Tipo y forma: Article (Published version)
Área (Departamento): Bioquímica y Biología Molecular (Departamento de Bioquímica y Biología Molecular y Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Exportado de SIDERAL (2017-06-12-09:14:23)
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Record created 2017-06-12, last modified 2017-06-12