Toll-like receptor 9 modifies intestinal serotonergic system.

Layunta, E. (Universidad de Zaragoza) ; Latorre, E. ; Forcén, R. ; Grasa, L. (Universidad de Zaragoza) ; Pardo, J. (Universidad de Zaragoza) ; Castro, M. ; Plaza, M.A. (Universidad de Zaragoza) ; Arruebo, M.P. (Universidad de Zaragoza) ; Mesonero, J.E. (Universidad de Zaragoza)

Resumen: Introduction: Toll-like receptor 9 (TLR9) is expressed in intestinal epithelial cells, which recognize microbiota developing different responses 1. Several studies have shown that TLR9 seems to be involved in Inflammatory Bowel Diseases (IBD) due to an inappropriate defensive response against microorganisms 2. Moreover, intestinal serotonergic system is also altered in IBD, where extracellular serotonin (5–HT) levels are increased 3. 5-HT bioavailability is mainly regulated by the serotonin transporter (SERT), expressed in enterocytes 4. Aims & Methods: The aim of the present study was to analyse whether TLR9 activation affects SERT expression and activity, and expression of other elements from the serotonergic system (TPH1, TPH2 and 5-HT receptors). Human enterocyte-like Caco-2 cells, and ileum and colon from TLR9-/- mice and Dextran Sulphate Sodium (DSS) mouse colitis model were used as experimental models. mRNA expression was determined by RT-qPCR, and protein expression by western blot...

Idioma: Inglés

DOI: 10.1177/2050640616663689

Año: 2016

Publicado en: United European Gastroenterology Journal 4, Suppl. 1 (2016), A426-A426
ISSN: 2050-6406

Factor impacto JCR: 3.673 (2016)

Categ. JCR: GASTROENTEROLOGY & HEPATOLOGY rank: 21 / 79 = 0.266 (2016) - Q2 - T1

Factor impacto SCIMAGO: 1.137 - Oncology (Q2) - Gastroenterology (Q2)

Tipo y forma: Article (Published version)

Área (Departamento): Área Fisiología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Área Inmunología (Dpto. Microb.Med.Pr.,Sal.Públ.)