000061644 001__ 61644
000061644 005__ 20200221144320.0
000061644 0247_ $$2doi$$a10.1089/ars.2014.6175
000061644 0248_ $$2sideral$$a99592
000061644 037__ $$aART-2016-99592
000061644 041__ $$aeng
000061644 100__ $$0(orcid)0000-0002-9312-1519$$aBotello-Morte,L
000061644 245__ $$aCysteine mutational studies provide insight into a thiol-based redox switch mechanism of metal and DNA binding in FurA from Anabaena sp. PCC 7120
000061644 260__ $$c2016
000061644 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061644 5203_ $$aAims: The ferric uptake regulator (Fur) is the main transcriptional regulator of genes involved in iron homeostasis in most prokaryotes. FurA from Anabaena sp. PCC 7120 contains five cysteine residues, four of them arranged in two redox-active CXXC motifs. The protein needs not only metal but also reducing conditions to remain fully active in vitro. Through a mutational study of the cysteine residues present in FurA, we have investigated their involvement in metal and DNA binding. Results: Residue C101 that belongs to a conserved CXXC motif plays an essential role in both metal and DNA binding activities in vitro. Substitution of C101 by serine impairs DNA and metal binding abilities of FurA. Isothermal titration calorimetry measurements show that the redox state of C101 is responsible for the protein ability to coordinate the metal corepressor. Moreover, the redox state of C101 varies with the presence or absence of C104 or C133, suggesting that the environments of these cysteines are mutually interdependent. Innovation: We propose that C101 is part of a thiol/disulfide redox switch that determines FurA ability to bind the metal corepressor. Conclusion: This mechanism supports a novel feature of a Fur protein that emerges as a regulator, which connects the response to changes in the intracellular redox state and iron management in cyanobacteria.
000061644 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B89$$9info:eu-repo/grantAgreement/ES/FIS/PI10-00186$$9info:eu-repo/grantAgreement/ES/ISCIII/CP07-00289$$9info:eu-repo/grantAgreement/ES/MICINN/BFU2010-19451$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458$$9info:eu-repo/grantAgreement/ES/MINECO/CSD2008-00005$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2011-24393
000061644 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061644 590__ $$a6.337$$b2016
000061644 591__ $$aENDOCRINOLOGY & METABOLISM$$b13 / 138 = 0.094$$c2016$$dQ1$$eT1
000061644 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b34 / 287 = 0.118$$c2016$$dQ1$$eT1
000061644 592__ $$a2.896$$b2016
000061644 593__ $$aBiochemistry$$c2016$$dQ1
000061644 593__ $$aCell Biology$$c2016$$dQ1
000061644 593__ $$aPhysiology$$c2016$$dQ1
000061644 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000061644 593__ $$aMolecular Biology$$c2016$$dQ1
000061644 593__ $$aClinical Biochemistry$$c2016$$dQ1
000061644 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061644 700__ $$aPellicer,S.
000061644 700__ $$aContreras,L. M.
000061644 700__ $$aNeira,J. L.
000061644 700__ $$0(orcid)0000-0001-5664-1729$$aAbian,O.$$uUniversidad de Zaragoza
000061644 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy,A.$$uUniversidad de Zaragoza
000061644 700__ $$0(orcid)0000-0002-2742-3711$$aPeleato,M. L.$$uUniversidad de Zaragoza
000061644 700__ $$0(orcid)0000-0001-8644-4574$$aFillat,M. F.$$uUniversidad de Zaragoza
000061644 700__ $$0(orcid)0000-0002-8181-2689$$aBes,M. T.$$uUniversidad de Zaragoza
000061644 7102_ $$11002$$2412$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Fisiología Vegetal
000061644 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000061644 773__ $$g24, 4 (2016), 173-185$$pAntioxid. redox signal.$$tANTIOXIDANTS & REDOX SIGNALING$$x1523-0864
000061644 8564_ $$s880572$$uhttps://zaguan.unizar.es/record/61644/files/texto_completo.pdf$$yVersión publicada
000061644 8564_ $$s116219$$uhttps://zaguan.unizar.es/record/61644/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061644 909CO $$ooai:zaguan.unizar.es:61644$$particulos$$pdriver
000061644 951__ $$a2020-02-21-13:41:16
000061644 980__ $$aARTICLE