000061832 001__ 61832
000061832 005__ 20170713115235.0
000061832 0247_ $$2doi$$a10.1111/apha.12498
000061832 0248_ $$2sideral$$a99902
000061832 037__ $$aART-2015-99902
000061832 041__ $$aeng
000061832 100__ $$aLatorre, E.
000061832 245__ $$aOxidative stress balance between pro- and anti-inflammatory factors in human intestinal epithelial cells
000061832 260__ $$c2015
000061832 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061832 5203_ $$aBackground: Oxidative stress plays a key role in the development of intestinal inflammatory diseases. Several pro-inflammatory mediators may generate oxidation products that exacerbate the inflammatory damage. Gastrointestinal molecules, like serotonin (5- HT), adenosine and melatonin, which are involved in intestinal physiology, have also been described as intestinal pro-inflammatory factors; whereas IL-10, a known anti-inflammatory cytokine, has also been implicated in intestinal pathophysiology. Aim: The aim of this study was to analyze the contribution of pro-inflammatory and anti-inflammatory molecules to oxidative stress balance, as well as to assess their effect on cellular antioxidant enzymes activity, in intestinal epithelial cells. Methods: Caco-2 cells were treated with the different molecules, and the oxidative stress was determined by measuring lipid peroxidation (MDA+4HDA) and protein carbonyl levels. The activity of the anti-oxidant enzymes (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) was also analyzed in treated cells. Results: Pro-inflammatory factors (5-HT, adenosine, melatonin and TNFa) increased oxidative damage in both lipids and proteins. Theses molecules, except melatonin, also inhibited the activity of antioxidant enzymes. With regard to IL-10, this cytokine was not shown to alter cellular oxidative damage, but was able to reduce the oxidative damage g by pro-inflamatory factors, and to restore their effects on anti-oxidant enxymes activities. Unexpectedly, IL-10, together with melatonin, was found to increase the antioxidant activity above the control. Conclusions: The anti-oxidant effect of IL-10 emphasizes the role of this cytokine as a potential therapy for the treatment of intestinal inflammation induced by pro-inflammatory molecules.
000061832 536__ $$9info:eu-repo/grantAgreement/ES/DGA/ARAINF-012-2008$$9info:eu-repo/grantAgreement/ES/DGA/B022-13$$9info:eu-repo/grantAgreement/ES/DGA/B105-11$$9info:eu-repo/grantAgreement/ES/DGA/B61$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2009-08149$$9info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971
000061832 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061832 590__ $$a4.066$$b2015
000061832 591__ $$aPHYSIOLOGY$$b13 / 83 = 0.157$$c2015$$dQ1$$eT1
000061832 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061832 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, E.$$uUniversidad de Zaragoza
000061832 700__ $$0(orcid)0000-0003-2373-6056$$aGimeno, A.$$uUniversidad de Zaragoza
000061832 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, L.$$uUniversidad de Zaragoza
000061832 700__ $$0(orcid)0000-0001-8584-3979$$aCastro, M.$$uUniversidad de Zaragoza
000061832 700__ $$aGarcía, S.
000061832 700__ $$aGros, B.
000061832 700__ $$0(orcid)0000-0002-2114-0577$$aArruebo, M.P.$$uUniversidad de Zaragoza
000061832 700__ $$aAlcalde, A.I.
000061832 700__ $$0(orcid)0000-0003-4758-3998$$aMesonero, J.E.$$uUniversidad de Zaragoza
000061832 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDepartamento de Farmacología y Fisiología$$cFisiología
000061832 773__ $$g214, Suppl. 701 (2015), 30 [14]$$pActa Physiol.$$tActa Physiologica$$x1748-1708
000061832 8564_ $$s36654$$uhttp://zaguan.unizar.es/record/61832/files/texto_completo.pdf$$yVersión publicada
000061832 8564_ $$s119323$$uhttp://zaguan.unizar.es/record/61832/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061832 909CO $$ooai:zaguan.unizar.es:61832$$particulos$$pdriver
000061832 951__ $$a2017-07-13-11:12:11
000061832 980__ $$aARTICLE