000061835 001__ 61835
000061835 005__ 20231215090947.0
000061835 0247_ $$2doi$$a10.1038/ncomms5450
000061835 0248_ $$2sideral$$a98809
000061835 037__ $$aART-2014-98809
000061835 041__ $$aeng
000061835 100__ $$aIto, S.
000061835 245__ $$aLoss of neuronal 3d chromatin organization causes transcriptional and behavioural deficits related to serotonergic dysfunction
000061835 260__ $$c2014
000061835 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061835 5203_ $$aThe interior of the neuronal cell nucleus is a highly organized three-dimensional (3D) structure where regions of the genome that are linearly millions of bases apart establish sub-structures with specialized functions. To investigate neuronal chromatin organization and dynamics in vivo, we generated bitransgenic mice expressing GFP-tagged histone H2B in principal neurons of the forebrain. Surprisingly, the expression of this chimeric histone in mature neurons caused chromocenter declustering and disrupted the association of heterochromatin with the nuclear lamina. The loss of these structures did not affect neuronal viability but was associated with specific transcriptional and behavioural deficits related to serotonergic dysfunction. Overall, our results demonstrate that the 3D organization of chromatin within neuronal cells provides an additional level of epigenetic regulation of gene expression that critically impacts neuronal function. This in turn suggests that some loci associated with neuropsychiatric disorders may be particularly sensitive to changes in chromatin architecture.
000061835 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000061835 590__ $$a11.47$$b2014
000061835 591__ $$aMULTIDISCIPLINARY SCIENCES$$b3 / 57 = 0.053$$c2014$$dQ1$$eT1
000061835 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061835 700__ $$aMagalska, A.
000061835 700__ $$aAlcaraz-Iborra, M.
000061835 700__ $$aLopez-Atalaya, J.
000061835 700__ $$aRovira, V.
000061835 700__ $$0(orcid)0000-0002-5462-907X$$aContreras-Moreira, B.
000061835 700__ $$aLipinski, M.
000061835 700__ $$aOlivares, R.
000061835 700__ $$aMartinez-Hernandez, J.
000061835 700__ $$aRuszczycki, B.
000061835 700__ $$aLujan, R.
000061835 700__ $$aGeijo-Barrientos, E.
000061835 700__ $$aWilczynski, G.M.
000061835 700__ $$aBarco, A.
000061835 773__ $$g5 (2014), 4450 [14 pp]$$tNature communications$$x2041-1723
000061835 8564_ $$s6188991$$uhttps://zaguan.unizar.es/record/61835/files/texto_completo.pdf$$yVersión publicada
000061835 8564_ $$s76857$$uhttps://zaguan.unizar.es/record/61835/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061835 909CO $$ooai:zaguan.unizar.es:61835$$particulos$$pdriver
000061835 951__ $$a2023-12-15-08:57:02
000061835 980__ $$aARTICLE