000061984 001__ 61984
000061984 005__ 20190709135432.0
000061984 0247_ $$2doi$$a10.3748/wjg.v23.i23.4211
000061984 0248_ $$2sideral$$a100975
000061984 037__ $$aART-2017-100975
000061984 041__ $$aeng
000061984 100__ $$aZaouali, Mohamed Amine.
000061984 245__ $$aRelevance of proteolysis and proteasome activation in fatty liver graft preservation: an Institut Georges Lopez-1 vs University of Wisconsin appraisal
000061984 260__ $$c2017
000061984 5060_ $$aAccess copy available to the general public$$fUnrestricted
000061984 5203_ $$aAIM To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) solutions. METHODS Fatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 °C and subjected to "ex vivo " normo-thermic perfusion (2 h; 37 °C). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3). RESULTS Profiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW (p < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 levels. CONCLUSION Our comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a higher UPS inhibition and reduced liver proteolysis.
000061984 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI12-0056
000061984 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttp://creativecommons.org/licenses/by-nc/3.0/es/
000061984 590__ $$a3.3$$b2017
000061984 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b35 / 80 = 0.438$$c2017$$dQ2$$eT2
000061984 592__ $$a1.409$$b2017
000061984 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000061984 593__ $$aGastroenterology$$c2017$$dQ1
000061984 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000061984 700__ $$aPanisello-Roselló, Arnau
000061984 700__ $$aLopez, Alexandre
000061984 700__ $$aCastro Benítez, Carlos
000061984 700__ $$aFolch-Puy, Emma
000061984 700__ $$0(orcid)0000-0002-8275-7191$$aGarcía-Gil, Agustín$$uUniversidad de Zaragoza
000061984 700__ $$aCarbonell, Teresa
000061984 700__ $$aAdam, René
000061984 700__ $$aRoselló-Catafau, Joan
000061984 7102_ $$11004$$2090$$aUniversidad de Zaragoza$$bDpto. Cirugía,Ginecol.Obstetr.$$cÁrea Cirugía
000061984 773__ $$g23, 23 (2017), 4211-4221$$tWORLD JOURNAL OF GASTROENTEROLOGY$$x1007-9327
000061984 8564_ $$s2033930$$uhttps://zaguan.unizar.es/record/61984/files/texto_completo.pdf$$yVersión publicada
000061984 8564_ $$s82730$$uhttps://zaguan.unizar.es/record/61984/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000061984 909CO $$ooai:zaguan.unizar.es:61984$$particulos$$pdriver
000061984 951__ $$a2019-07-09-11:32:27
000061984 980__ $$aARTICLE