000062016 001__ 62016 000062016 005__ 20190709135434.0 000062016 0248_ $$2sideral$$a101067 000062016 037__ $$aART-2017-101067 000062016 041__ $$aeng 000062016 100__ $$aQuiñones, J. 000062016 245__ $$aGenetic polymorphism in meat fatty acids in araucano creole sheeps 000062016 260__ $$c2017 000062016 5060_ $$aAccess copy available to the general public$$fUnrestricted 000062016 5203_ $$aMeat is a source of proteins and minerals. However, red meats have high levels of saturated fatty acids (SFA) and a low proportion of monounsaturated fatty acids (MUFA), a combination which has been linked to cancer and cardiovascular diseases. In ruminants, there are several genes that regulate the proportions of MUFA in tissues, but the most important is SCD (Stearoyl-CoA desaturase). The polymorphism g.31C >A has been described in the promoter region of the SCD gene, which is associated with changes in the gene expression and MUFA levels in the meat. The aim of this study was to detect the presence of polymorphism g.31C >A in a population of Araucano creole sheep using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Genomic DNA was obtained from 157 Araucano creole sheep. The SCD promoter region was amplified using PCR and the amplicons were digested with restriction enzyme MnlI. The allelic frequency was 0.98 for the C allele and 0.02 for the A allele. The in silico analysis showed that the A allele could alter the expression of SCD. This is the first report of the presence of polymorphism g.31C >A in Chilean sheep, and its association with SCD expression levels and the proportion of MUFA in the meat will require further investigation. 000062016 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000062016 590__ $$a0.407$$b2017 000062016 591__ $$aAGRICULTURE, MULTIDISCIPLINARY$$b42 / 56 = 0.75$$c2017$$dQ3$$eT3 000062016 591__ $$aVETERINARY SCIENCES$$b111 / 140 = 0.793$$c2017$$dQ4$$eT3 000062016 592__ $$a0.272$$b2017 000062016 593__ $$aPlant Science$$c2017$$dQ3 000062016 593__ $$aAnimal Science and Zoology$$c2017$$dQ3 000062016 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000062016 700__ $$aBravo, S. 000062016 700__ $$0(orcid)0000-0001-9513-0219$$aCalvo, J.H. 000062016 700__ $$aSepúlveda, N. 000062016 773__ $$g27, 3 (2017), 743-746$$pJOURNAL OF ANIMAL AND PLANT SCIENCES$$tJOURNAL OF ANIMAL AND PLANT SCIENCES$$x1018-7081 000062016 85641 $$uhttp://www.thejaps.org.pk/docs/v-27-03/07.pdf$$zTexto completo de la revista 000062016 8564_ $$s201700$$uhttps://zaguan.unizar.es/record/62016/files/texto_completo.pdf$$yVersión publicada 000062016 8564_ $$s123538$$uhttps://zaguan.unizar.es/record/62016/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000062016 909CO $$ooai:zaguan.unizar.es:62016$$particulos$$pdriver 000062016 951__ $$a2019-07-09-11:33:25 000062016 980__ $$aARTICLE