000062042 001__ 62042
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000062042 0247_ $$2doi$$a10.17235/reed.2015.3868/2015
000062042 0248_ $$2sideral$$a93221
000062042 037__ $$aART-2015-93221
000062042 041__ $$aeng
000062042 100__ $$aNevado, R.
000062042 245__ $$aNeomycin and bacitracin reduce the intestinal permeability in mice and increase the expression of some tight-junction proteins
000062042 260__ $$c2015
000062042 5060_ $$aAccess copy available to the general public$$fUnrestricted
000062042 5203_ $$aBackground: Tight-junction (TJ) proteins regulate paracellular permeability. Gut permeability can be modulated by commensal microbiota. Manipulation of the gut microbiota with antibiotics like bacitracin and neomycin turned out to be useful for the treatment of diarrhoea induced by Clostridium difficile or chemotherapy drugs. Aim: To evaluate the effects of the microbiota depletion evoked by the oral administration of neomycin and bacitracin on the intestinal permeability and expression of TJ proteins in mice. Methods: Mice received neomycin and bacitracin orally for 7 days. Intestinal permeability was measured by the fluoresceinisothiocyanate- dextran (FITC-dextran) method. The gene expression of TJ proteins in the intestine was determined by real time-PCR. Results: FITC-dextran levels in serum were reduced by half in antibiotic-treated mice, indicating a reduction of intestinal permeability. Antibiotics increased the expression of zonula occludens 1 (ZO-1), junctional adhesion molecule A (JAM-A, and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon. Conclusion: The combination of neomycin and bacitracin reduce intestinal permeability and increase the gene expression of ZO-1, junctional adhesion molecule A (JAM-A), and occludin in the ileum and ZO-1, claudin-3, and claudin-4 in the colon.
000062042 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B61$$9info:eu-repo/grantAgreement/ES/UZ/JIUZ-2013-BIO-08
000062042 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000062042 590__ $$a1.455$$b2015
000062042 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b67 / 79 = 0.848$$c2015$$dQ4$$eT3
000062042 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000062042 700__ $$aForcén, R.
000062042 700__ $$0(orcid)0000-0001-5573-6144$$aLayunta, E.$$uUniversidad de Zaragoza
000062042 700__ $$0(orcid)0000-0002-3917-4740$$aMurillo, M. D.
000062042 700__ $$0(orcid)0000-0002-5306-9365$$aGrasa, L.$$uUniversidad de Zaragoza
000062042 7102_ $$11005$$2410$$aUniversidad de Zaragoza$$bDepartamento de Farmacología y Fisiología$$cFisiología
000062042 773__ $$g107, 11 (2015), 672-676$$pRev. esp. enferm. dig.$$tREVISTA ESPANOLA DE ENFERMEDADES DIGESTIVAS$$x1130-0108
000062042 85641 $$uhttps:; online.reed.es/DOI/PDF/ArticuloDOI_3868.pdf$$zTexto completo de la revista
000062042 8564_ $$s722974$$uhttp://zaguan.unizar.es/record/62042/files/texto_completo.pdf$$yVersión publicada
000062042 8564_ $$s111001$$uhttp://zaguan.unizar.es/record/62042/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000062042 909CO $$ooai:zaguan.unizar.es:62042$$particulos$$pdriver
000062042 951__ $$a2017-09-11-14:08:35
000062042 980__ $$aARTICLE