000063162 001__ 63162
000063162 005__ 20190529115222.0
000063162 0247_ $$2doi$$a10.1186/1743-8977-11-4
000063162 0248_ $$2sideral$$a85377
000063162 037__ $$aART-2014-85377
000063162 041__ $$aeng
000063162 100__ $$aNymark, P.
000063162 245__ $$aFree radical scavenging and formation by multi-walled carbon nanotubes in cell free conditions and in human bronchial epithelial cells
000063162 260__ $$c2014
000063162 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063162 5203_ $$aBackground:
Certain multi-walled carbon nanotubes (MWCNTs) have been shown to elicit asbestos-like toxicological effects. To reduce needs for risk assessment it has been suggested that the physicochemical characteristics or reactivity of nanomaterials could be used to predict their hazard. Fibre-shape and ability to generate reactive oxygen species (ROS) are important indicators of high hazard materials. Asbestos is a known ROS generator, while MWCNTs may either produce or scavenge ROS. However, certain biomolecules, such as albumin – used as dispersants in nanomaterial preparation for toxicological testing in vivo and in vitro - may reduce the surface reactivity of nanomaterials.
Methods:
Here, we investigated the effect of bovine serum albumin (BSA) and cell culture medium with and without BEAS 2B cells on radical formation/scavenging by five MWCNTs, Printex 90 carbon black, crocidolite asbestos, and glass wool, using electron spin resonance (ESR) spectroscopy and linked this to cytotoxic effects measured by trypan blue exclusion assay. In addition, the materials were characterized in the exposure medium (e.g. for hydrodynamic size-distribution and sedimentation rate).
Results:
The test materials induced highly variable cytotoxic effects which could generally be related to the abundance and characteristics of agglomerates/aggregates and to the rate of sedimentation. All carbon nanomaterials were found to scavenge hydroxyl radicals (•OH) in at least one of the solutions tested. The effect of BSA was different among the materials. Two types of long, needle-like MWCNTs (average diameter >74 and 64.2 nm, average length 5.7 and 4.0 µm, respectively) induced, in addition to a scavenging effect, a dose-dependent formation of a unique, yet unidentified radical in both absence and presence of cells, which also coincided with cytotoxicity.
Conclusions:
Culture medium and BSA affects scavenging/production of •OH by MWCNTs, Printex 90 carbon black, asbestos and glass-wool. An unidentified radical is generated by two long, needle-like MWCNTs and these two CNTs were more cytotoxic than the other CNTs tested, suggesting that this radical could be related to the adverse effects of MWCNTs.
000063162 536__ $$9info:eu-repo/grantAgreement/EC/FP7/299525/EU/Toxicogenomic studies on engineered carbon nanomaterials/miRNAno
000063162 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000063162 590__ $$a7.113$$b2014
000063162 591__ $$aTOXICOLOGY$$b3 / 88 = 0.034$$c2014$$dQ1$$eT1
000063162 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063162 700__ $$aAlstrup, J. K.
000063162 700__ $$aSuhonen, S.
000063162 700__ $$aKembouche, Y.
000063162 700__ $$aVippola, M.
000063162 700__ $$aKleinjans, J.
000063162 700__ $$0(orcid)0000-0003-2936-242X$$aCatalán, J.$$uUniversidad de Zaragoza
000063162 700__ $$aNorppa, H.
000063162 700__ $$avan Delft, J.
000063162 700__ $$aBriedé, J. J.
000063162 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000063162 773__ $$g11, 4 (2014), [18 pp]$$pPart fibre toxicol.$$tPARTICLE AND FIBRE TOXICOLOGY$$x1743-8977
000063162 8564_ $$s5270007$$uhttps://zaguan.unizar.es/record/63162/files/texto_completo.pdf$$yVersión publicada
000063162 8564_ $$s90146$$uhttps://zaguan.unizar.es/record/63162/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063162 909CO $$ooai:zaguan.unizar.es:63162$$particulos$$pdriver
000063162 951__ $$a2019-05-29-11:42:18
000063162 980__ $$aARTICLE