Multinuclear nanoliter one-dimensional and two-dimensional NMR spectroscopy with a single non-resonant microcoil

Fratila, Raluca M. (Universidad de Zaragoza) ; Gomez, M. Victoria ; Sykora, Stanislav ; Velders, Aldrik H.

Resumen: Nuclear magnetic resonance (NMR) spectroscopy is a powerful analytical technique, but its low sensitivity and highly sophisticated, costly, equipment severely constrain more widespread applications. Here we show that a non-resonant planar transceiver microcoil integrated in a microfluidic chip (detection volume 25¿nl) can detect different nuclides in the full broad-band range of Larmor frequencies (at 9.4¿T from 61 to 400¿MHz). Routine one-dimensional (1D) and two-dimensional (2D), homo- and heteronuclear experiments can be carried out using the broad-band coil set-up. Noteworthy, heteronuclear 2D experiments can be performed in a straightforward manner on virtually any combination of nuclides (from classical 1H–13C to more exotic combinations like 19F–31P) both in coupled and decoupled mode. Importantly, the concept of a non-resonant system provides magnetic field-independent NMR probes; moreover, the small-volume alleviates problems related to field inhomogeneity, making the broad-band coil an attractive option for, for example, portable and table-top NMR systems.

Idioma: Inglés

DOI: 10.1038/ncomms4025

Año: 2014

Publicado en: NATURE COMMUNICATIONS 5, 3025 (2014), 1-8
ISSN: 2041-1723

Factor impacto JCR: 11.47 (2014)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 3 / 57 = 0.053 (2014) - Q1 - T1

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Química Orgánica (Departamento de Química Orgánica)