000063301 001__ 63301
000063301 005__ 20201021165702.0
000063301 0247_ $$2doi$$a10.1371/journal.pone.0061118
000063301 0248_ $$2sideral$$a81230
000063301 037__ $$aART-2013-81230
000063301 041__ $$aeng
000063301 100__ $$0(orcid)0000-0001-5105-6133$$aAcín, C.$$uUniversidad de Zaragoza
000063301 245__ $$aPrion Protein Gene Variability in Spanish Goats. Inference through Susceptibility to Classical Scrapie Strains and Pathogenic Distribution of Peripheral PrPsc
000063301 260__ $$c2013
000063301 5060_ $$aAccess copy available to the general public$$fUnrestricted
000063301 5203_ $$aClassical scrapie is a neurological disorder of the central nervous system (CNS) characterized by the accumulation of an abnormal, partially protease resistant prion protein (PrPsc) in the CNS and in some peripheral tissues in domestic small ruminants. Whereas the pathological changes and genetic susceptibility of ovine scrapie are well known, caprine scrapie has been less well studied. We report here a pathological study of 13 scrapie-affected goats diagnosed in Spain during the last 9 years. We used immunohistochemical and biochemical techniques to discriminate between classical and atypical scrapie and bovine spongiform encephalopathy (BSE). All the animals displayed PrPsc distribution patterns and western blot characteristics compatible with classical scrapie. In addition, we determined the complete open reading frame sequence of the PRNP in these scrapie-affected animals. The polymorphisms observed were compared with those of the herd mates (n¿=¿665) and with the frequencies of healthy herds (n¿=¿581) of native Spanish goats (Retinta, Pirenaica and Moncaina) and other worldwide breeds reared in Spain (Saanen, Alpine and crossbreed). In total, sixteen polymorphic sites were identified, including the known amino acid substitutions at codons G37V, G127S, M137I, I142M, H143R, R151H, R154H, R211Q, Q222K, G232W, and P240S, and new polymorphisms at codons G74D, M112T, R139S, L141F and Q215R. In addition, the known 42, 138 and 179 silent mutations were detected, and one new one is reported at codon 122. The genetic differences observed in the population studied have been attributed to breed and most of the novel polymorphic codons show frequencies lower than 5%. This work provides the first basis of polymorphic distribution of PRNP in native and worldwide goat breeds reared in Spain.
000063301 536__ $$9info:eu-repo/grantAgreement/ES/CICYT/EET-2001-4033$$9info:eu-repo/grantAgreement/EUR/FP6/FOODCT-2006-36353-Goat-BSE
000063301 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000063301 590__ $$a3.534$$b2013
000063301 591__ $$aMULTIDISCIPLINARY SCIENCES$$b8 / 56 = 0.143$$c2013$$dQ1$$eT1
000063301 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000063301 700__ $$0(orcid)0000-0001-6016-4726$$aMartín-Burriel, I.$$uUniversidad de Zaragoza
000063301 700__ $$0(orcid)0000-0002-7453-1766$$aMonleón, E.$$uUniversidad de Zaragoza
000063301 700__ $$0(orcid)0000-0003-4796-1872$$aLyahyai, J.
000063301 700__ $$0(orcid)0000-0003-3016-9125$$aPitarch, J.L.$$uUniversidad de Zaragoza
000063301 700__ $$aSerrano, C.
000063301 700__ $$0(orcid)0000-0002-2787-9671$$aMonzón, M.$$uUniversidad de Zaragoza
000063301 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza
000063301 700__ $$0(orcid)0000-0002-7173-7216$$aBadiola, J.J.$$uUniversidad de Zaragoza
000063301 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000063301 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000063301 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000063301 773__ $$g8, 4 (2013), e61118 [13 pp]$$pPLoS One$$tPloS one$$x1932-6203
000063301 8564_ $$s457994$$uhttps://zaguan.unizar.es/record/63301/files/texto_completo.pdf$$yVersión publicada
000063301 8564_ $$s127994$$uhttps://zaguan.unizar.es/record/63301/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000063301 909CO $$ooai:zaguan.unizar.es:63301$$particulos$$pdriver
000063301 951__ $$a2020-10-21-16:49:09
000063301 980__ $$aARTICLE