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000064360 0247_ $$2doi$$a10.1039/c6cp07508h
000064360 0248_ $$2sideral$$a101122
000064360 037__ $$aART-2017-101122
000064360 041__ $$aeng
000064360 100__ $$0(orcid)0000-0001-5072-0645$$aTapia-Rojo, Rafael
000064360 245__ $$aA physical picture for mechanical dissociation of biological complexes: From forces to free energies
000064360 260__ $$c2017
000064360 5060_ $$aAccess copy available to the general public$$fUnrestricted
000064360 5203_ $$aSingle-molecule force spectroscopy is a powerful technique based on the application of controlled forces to macromolecules. In order to relate the measured response of the molecule to its equilibrium and dynamic properties, a suitable physical picture of the involved process is necessary. In this work, we introduce a plausible model for mechanical unbinding of some molecular complexes, based on a novel free energy profile. We combine two standard theoretical frameworks for analyzing force spectroscopy experiments on two protein:protein complexes, obtaining key magnitudes of the underlying free energy profile, which are only understood within the mentioned model. Additionally, we carry out detailed stochastic dynamics simulations to prove the validity of the analysis protocol and the reliability of the free energy profile. Remarkably, we can compare directly the obtained unbinding free energies with the previously known bulk binding free energies, bridging the gap between bulk and single molecule techniques.
000064360 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BIO2013-42978-P$$9info:eu-repo/grantAgreement/ES/MINECO/FIS2011-25167$$9info:eu-repo/grantAgreement/ES/MINECO/FIS2014-55867$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2012-35358
000064360 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000064360 590__ $$a3.906$$b2017
000064360 591__ $$aPHYSICS, ATOMIC, MOLECULAR & CHEMICAL$$b9 / 36 = 0.25$$c2017$$dQ1$$eT1
000064360 591__ $$aCHEMISTRY, PHYSICAL$$b46 / 146 = 0.315$$c2017$$dQ2$$eT1
000064360 592__ $$a1.686$$b2017
000064360 593__ $$aPhysics and Astronomy (miscellaneous)$$c2017$$dQ1
000064360 593__ $$aPhysical and Theoretical Chemistry$$c2017$$dQ1
000064360 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000064360 700__ $$0(orcid)0000-0003-3459-8605$$aMarcuello, Carlos
000064360 700__ $$0(orcid)0000-0001-7460-5916$$aLostao, Anabel$$uUniversidad de Zaragoza
000064360 700__ $$0(orcid)0000-0003-4087-4733$$aGómez-Moreno, Carlos$$uUniversidad de Zaragoza
000064360 700__ $$0(orcid)0000-0003-0698-6555$$aMazo, Juan J.$$uUniversidad de Zaragoza
000064360 700__ $$0(orcid)0000-0002-9551-624X$$aFalo, Fernando$$uUniversidad de Zaragoza
000064360 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000064360 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000064360 773__ $$g19, 6 (2017), 4567-4575$$pPhys. chem. chem. phys.$$tPHYSICAL CHEMISTRY CHEMICAL PHYSICS$$x1463-9076
000064360 8564_ $$s4338200$$uhttps://zaguan.unizar.es/record/64360/files/texto_completo.pdf$$yPostprint
000064360 8564_ $$s104518$$uhttps://zaguan.unizar.es/record/64360/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000064360 909CO $$ooai:zaguan.unizar.es:64360$$particulos$$pdriver
000064360 951__ $$a2023-03-13-13:54:29
000064360 980__ $$aARTICLE