000064363 001__ 64363
000064363 005__ 20190709135518.0
000064363 0247_ $$2doi$$a10.1515/cclm-2016-0690
000064363 0248_ $$2sideral$$a100951
000064363 037__ $$aART-2017-100951
000064363 041__ $$aeng
000064363 100__ $$aGarcía-González, Elena
000064363 245__ $$aDetecting paraprotein interference on a direct bilirubin assay by reviewing the photometric reaction data
000064363 260__ $$c2017
000064363 5060_ $$aAccess copy available to the general public$$fUnrestricted
000064363 5203_ $$aThe direct bilirubin (D-Bil) assay on the AU Beckman Coulter instrumentation can be interfered by paraproteins, which may result in spurious D-Bil results. In a previous work, we took advantage of this fact to detect this interference, thus helping with the identification of patients with unsuspected monoclonal gammopathies. In this work, we investigate the possibility to detect interference based on the review of the photometric reactions, regardless of the D-Bil result. The D-Bil assay was carried out in a set of 2164 samples. It included a group of 164 samples with paraproteins (67 of which caused interference on the assay), as well as different groups of samples for which high absorbance background readings could also be expected (i.e. hemolyzed, lipemic, or icteric samples). Photometric reaction data were reviewed and receiver operating characteristics (ROC) curves were used to establish a cut-off for absorbance that best discriminates interference. The best cut-off was 0.0100 for the absorbance at the first photometric point of the complementary wavelength in the blank cuvette. Once the optimal cut-off for probable interference was selected, all samples analyzed in our laboratory that provided absorbance values above this cut-off were further investigated to try to discover paraproteins. During a period of 6 months, we detected 44 samples containing paraproteins, five of which belonged to patients with non-diagnosed monoclonal gammopathies. Review of the photometric reaction data permits the systematic detection of paraprotein interference on the D-Bil AU assay, even for samples for which reasonable results are obtained.
000064363 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64684-P
000064363 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000064363 590__ $$a3.556$$b2017
000064363 591__ $$aMEDICAL LABORATORY TECHNOLOGY$$b7 / 30 = 0.233$$c2017$$dQ1$$eT1
000064363 592__ $$a1.114$$b2017
000064363 593__ $$aBiochemistry (medical)$$c2017$$dQ1
000064363 593__ $$aMedicine (miscellaneous)$$c2017$$dQ1
000064363 593__ $$aClinical Biochemistry$$c2017$$dQ2
000064363 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000064363 700__ $$0(orcid)0000-0002-3916-9992$$aAramendía, Maite
000064363 700__ $$aGonzález-Tarancón, Ricardo
000064363 700__ $$aRomero-Sánchez, Naiara
000064363 700__ $$aRello, Luis
000064363 773__ $$g55, 8 (2017), 1178-1185$$pClin. chem. lab. med.$$tClinical Chemistry and Laboratory Medicine$$x1434-6621
000064363 8564_ $$s223625$$uhttps://zaguan.unizar.es/record/64363/files/texto_completo.pdf$$yVersión publicada
000064363 8564_ $$s122004$$uhttps://zaguan.unizar.es/record/64363/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000064363 909CO $$ooai:zaguan.unizar.es:64363$$particulos$$pdriver
000064363 951__ $$a2019-07-09-11:56:12
000064363 980__ $$aARTICLE