000065257 001__ 65257
000065257 005__ 20200117211601.0
000065257 0247_ $$2doi$$a10.3389/fmicb.2017.02641
000065257 0248_ $$2sideral$$a104191
000065257 037__ $$aART-2018-104191
000065257 041__ $$aeng
000065257 100__ $$aDomínguez-Martín, M.A.
000065257 245__ $$aDifferential NtcA responsiveness to 2-oxoglutarate underlies the diversity of C/N balance regulation in Prochlorococcus
000065257 260__ $$c2018
000065257 5060_ $$aAccess copy available to the general public$$fUnrestricted
000065257 5203_ $$aPrevious studies showed differences in the regulatory response to C/N balance in Prochlorococcus with respect to other cyanobacteria, but no information was available about its causes, or the ecological advantages conferred to thrive in oligotrophic environments. We addressed the changes in key enzymes (glutamine synthetase, isocitrate dehydrogenase) and the ntcA gene (the global nitrogen regulator) involved in C/N metabolism and its regulation, in three model Prochlorococcus strains: MED4, SS120, and MIT9313. We observed a remarkable level of diversity in their response to azaserine, a glutamate synthase inhibitor which increases the concentration of the key metabolite 2-oxoglutarate, used to sense the C/N balance by cyanobacteria. Besides, we studied the binding between the global nitrogen regulator (NtcA) and the promoter of the glnA gene in the same Prochlorococcus strains, and its dependence on the 2-oxoglutarate concentration, by using isothermal titration calorimetry, surface plasmon resonance, and electrophoretic mobility shift. Our results show a reduction in the responsiveness of NtcA to 2-oxoglutarate in Prochlorococcus, especially in the MED4 and SS120 strains. This suggests a trend to streamline the regulation of C/N metabolism in late-branching Prochlorococcus strains (MED4 and SS120), in adaptation to the rather stable conditions found in the oligotrophic ocean gyres where this microorganism is most abundant.
000065257 536__ $$9info:eu-repo/grantAgreement/ES/FIS/PI15-00663$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2013-44767-P$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2013-47064-P$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-76227-P$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P
000065257 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000065257 590__ $$a4.259$$b2018
000065257 591__ $$aMICROBIOLOGY$$b32 / 133 = 0.241$$c2018$$dQ1$$eT1
000065257 592__ $$a1.633$$b2018
000065257 593__ $$aMicrobiology (medical)$$c2018$$dQ1
000065257 593__ $$aMicrobiology$$c2018$$dQ1
000065257 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000065257 700__ $$aLópez-Lozano, A.
000065257 700__ $$aClavería-Gimeno, R.
000065257 700__ $$0(orcid)0000-0001-5702-4538$$aVelázquez-Campoy, A.$$uUniversidad de Zaragoza
000065257 700__ $$aSeidel, G.
000065257 700__ $$aBurkovski, A.
000065257 700__ $$aDíez, J.
000065257 700__ $$aGarcía-Fernández, J.M.
000065257 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000065257 773__ $$g8, JAN (2018), 2641 [16 pp]$$pFront. microbiol.$$tFRONTIERS IN MICROBIOLOGY$$x1664-302X
000065257 8564_ $$s2505479$$uhttps://zaguan.unizar.es/record/65257/files/texto_completo.pdf$$yVersión publicada
000065257 8564_ $$s11477$$uhttps://zaguan.unizar.es/record/65257/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000065257 909CO $$ooai:zaguan.unizar.es:65257$$particulos$$pdriver
000065257 951__ $$a2020-01-17-21:12:26
000065257 980__ $$aARTICLE