000065271 001__ 65271
000065271 005__ 20201130083155.0
000065271 0247_ $$2doi$$a10.3390/molecules23010029
000065271 0248_ $$2sideral$$a104238
000065271 037__ $$aART-2018-104238
000065271 041__ $$aeng
000065271 100__ $$0(orcid)0000-0001-9047-0046$$aMartínez-Júlvez, M.$$uUniversidad de Zaragoza
000065271 245__ $$aIdentification of inhibitors targeting ferredoxin-NADP+ reductase from the xanthomonas citri subsp. Citri phytopathogenic bacteria
000065271 260__ $$c2018
000065271 5060_ $$aAccess copy available to the general public$$fUnrestricted
000065271 5203_ $$aFerredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials.
000065271 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B18$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2016-75183-P
000065271 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000065271 590__ $$a3.06$$b2018
000065271 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b67 / 172 = 0.39$$c2018$$dQ2$$eT2
000065271 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b133 / 294 = 0.452$$c2018$$dQ2$$eT2
000065271 592__ $$a0.757$$b2018
000065271 593__ $$aAnalytical Chemistry$$c2018$$dQ1
000065271 593__ $$aChemistry (miscellaneous)$$c2018$$dQ1
000065271 593__ $$aDrug Discovery$$c2018$$dQ1
000065271 593__ $$aPhysical and Theoretical Chemistry$$c2018$$dQ1
000065271 593__ $$aMolecular Medicine$$c2018$$dQ1
000065271 593__ $$aOrganic Chemistry$$c2018$$dQ1
000065271 593__ $$aPharmaceutical Science$$c2018$$dQ1
000065271 593__ $$aMedicine (miscellaneous)$$c2018$$dQ1
000065271 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000065271 700__ $$0(orcid)0000-0003-4010-849X$$aGoñi, G.
000065271 700__ $$aPérez-Amigot, D.
000065271 700__ $$aLaplaza, R.
000065271 700__ $$aIonescu, I.A.
000065271 700__ $$aPetrocelli, S.
000065271 700__ $$aTondo, M.L.
000065271 700__ $$0(orcid)0000-0002-2879-9200$$aSancho, J.$$uUniversidad de Zaragoza
000065271 700__ $$aOrellano, E.G.
000065271 700__ $$0(orcid)0000-0001-8743-0182$$aMedina, M.$$uUniversidad de Zaragoza
000065271 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000065271 773__ $$g23, 1 (2018), 29 [15 pp]$$pMolecules$$tMolecules$$x1420-3049
000065271 8564_ $$s3840426$$uhttps://zaguan.unizar.es/record/65271/files/texto_completo.pdf$$yVersión publicada
000065271 8564_ $$s104731$$uhttps://zaguan.unizar.es/record/65271/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000065271 909CO $$ooai:zaguan.unizar.es:65271$$particulos$$pdriver
000065271 951__ $$a2020-11-30-07:57:24
000065271 980__ $$aARTICLE