000065282 001__ 65282
000065282 005__ 20190709135644.0
000065282 0247_ $$2doi$$a10.1021/jacs.7b09447
000065282 0248_ $$2sideral$$a104258
000065282 037__ $$aART-2017-104258
000065282 041__ $$aeng
000065282 100__ $$aSomovilla, V.J.
000065282 245__ $$aThe Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer
000065282 260__ $$c2017
000065282 5060_ $$aAccess copy available to the general public$$fUnrestricted
000065282 5203_ $$aA structure-based design of a new generation of tumor-associated glycopeptides with improved affinity against two anti-MUC1 antibodies is described. These unique antigens feature a fluorinated proline residue, such as a (4S)-4-fluoro-l-proline or 4, 4-difluoro-l-proline, at the most immunogenic domain. Binding assays using biolayer interferometry reveal 3-fold to 10-fold affinity improvement with respect to the natural (glyco)peptides. According to X-ray crystallography and MD simulations, the fluorinated residues stabilize the antigen-antibody complex by enhancing key CH/p interactions. Interestingly, a notable improvement in detection of cancer-associated anti-MUC1 antibodies from serum of patients with prostate cancer is achieved with the non-natural antigens, which proves that these derivatives can be considered better diagnostic tools than the natural antigen for prostate cancer.
000065282 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/UNLR13-4E-1931$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-67727-R$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64597-C2-1-P$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2013-44367-C2-2-P$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-75633-P$$9info:eu-repo/grantAgreement/EC/FP7/283570/EU/Transnational access and enhancement of integrated Biological Structure determination at synchrotron X-ray radiation facilities/BIOSTRUCT-X$$9info:eu-repo/grantAgreement/ES/DGA/B89
000065282 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000065282 590__ $$a14.357$$b2017
000065282 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b8 / 171 = 0.047$$c2017$$dQ1$$eT1
000065282 592__ $$a8.127$$b2017
000065282 593__ $$aBiochemistry$$c2017$$dQ1
000065282 593__ $$aColloid and Surface Chemistry$$c2017$$dQ1
000065282 593__ $$aChemistry (miscellaneous)$$c2017$$dQ1
000065282 593__ $$aCatalysis$$c2017$$dQ1
000065282 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000065282 700__ $$aBermejo, I.A.
000065282 700__ $$aAlbuquerque, I.S.
000065282 700__ $$aMartínez-Sáez, N.
000065282 700__ $$aCastro-López, J.
000065282 700__ $$aGarcía-Martín, F.
000065282 700__ $$aCompañón, I.
000065282 700__ $$aHinou, H.
000065282 700__ $$aNishimura, S.-I.
000065282 700__ $$aJiménez-Barbero, J.
000065282 700__ $$aAsensio, J.L.
000065282 700__ $$aAvenoza, A.
000065282 700__ $$aBusto, J.H.
000065282 700__ $$0(orcid)0000-0002-3122-9401$$aHurtado-Guerrero, R.$$uUniversidad de Zaragoza
000065282 700__ $$aPeregrina, J.M.
000065282 700__ $$aBernardes, G.J.L.
000065282 700__ $$aCorzana, F.
000065282 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000065282 773__ $$g139, 50 (2017), 18255-18261$$pJ. Am. Chem. Soc.$$tJOURNAL OF THE AMERICAN CHEMICAL SOCIETY$$x0002-7863
000065282 8564_ $$s539769$$uhttps://zaguan.unizar.es/record/65282/files/texto_completo.pdf$$yVersión publicada
000065282 8564_ $$s120405$$uhttps://zaguan.unizar.es/record/65282/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000065282 909CO $$ooai:zaguan.unizar.es:65282$$particulos$$pdriver
000065282 951__ $$a2019-07-09-12:40:28
000065282 980__ $$aARTICLE