000065321 001__ 65321
000065321 005__ 20190709135643.0
000065321 0247_ $$2doi$$a10.3389/fimmu.2017.01817
000065321 0248_ $$2sideral$$a104253
000065321 037__ $$aART-2017-104253
000065321 041__ $$aeng
000065321 100__ $$aNúñez, D.
000065321 245__ $$aA functional analysis on the interspecies interaction between mouse LFA-1 and human intercellular adhesion molecule-1 at the cell level
000065321 260__ $$c2017
000065321 5060_ $$aAccess copy available to the general public$$fUnrestricted
000065321 5203_ $$aThe interaction between intercellular adhesion molecules (ICAM) and the integrin leukocyte function-associated antigen-1 (LFA-1) is crucial for the regulation of several physiological and pathophysiological processes like cell-mediated elimination of tumor or virus infected cells, cancer metastasis, or inflammatory and autoimmune processes. Using purified proteins it was reported a species restriction for the interaction of ICAM-1 and LFA-1, being mouse ICAM-1 able to interact with human LFA-1 but not human ICAM-1 with mouse LFA-1. However, in vivo results employing tumor cells transfected with human ICAM-1 suggest that functionally mouse LFA-1 can recognize human ICAM-1. In order to clarify the interspecies cross-reactivity of the ICAM-1/LFA-1 interaction, we have performed functional studies analyzing the ability of human soluble ICAM-1 and human/mouse LFA-1 derived peptides to inhibit cell aggregation and adhesion as well as cell-mediated cytotoxicity in both mouse and human systems. In parallel, the affinity of the interaction between mouse LFA-1-derived peptides and human ICAM-1 was determined by calorimetry assays. According to the results obtained, it seems that human ICAM-1 is able to interact with mouse LFA-1 on intact cells, which should be taking into account when using humanized mice and xenograft models for the study of immune-related processes.
000065321 536__ $$9info:eu-repo/grantAgreement/ES/DGA/FSE$$9info:eu-repo/grantAgreement/ES/MINECO/BFU2016-78232-P$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/SAF2011-25390$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-1-R$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2014-54763-C2-2-R
000065321 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000065321 590__ $$a5.511$$b2017
000065321 591__ $$aIMMUNOLOGY$$b30 / 155 = 0.194$$c2017$$dQ1$$eT1
000065321 592__ $$a2.803$$b2017
000065321 593__ $$aImmunology and Allergy$$c2017$$dQ1
000065321 593__ $$aImmunology$$c2017$$dQ1
000065321 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000065321 700__ $$aComas, L.
000065321 700__ $$0(orcid)0000-0001-7328-2094$$aLanuza, P.M.$$uUniversidad de Zaragoza
000065321 700__ $$aSánchez-Martinez, D.
000065321 700__ $$aPérez-Hernández, M.
000065321 700__ $$aCatalán, E.
000065321 700__ $$aDomingo, M.P.
000065321 700__ $$0(orcid)0000-0001-5702-4538$$aVelázquez-Campoy, A.$$uUniversidad de Zaragoza
000065321 700__ $$0(orcid)0000-0003-0154-0730$$aPardo, J.$$uUniversidad de Zaragoza
000065321 700__ $$aGálvez, E.M.
000065321 7102_ $$11008$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Med.Pr.,Sal.Públ.$$cÁrea Inmunología
000065321 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000065321 773__ $$g8, DEC (2017), 817 [12 pp]$$pFront. immunol.$$tFrontiers in Immunology$$x1664-3224
000065321 8564_ $$s334547$$uhttps://zaguan.unizar.es/record/65321/files/texto_completo.pdf$$yVersión publicada
000065321 8564_ $$s102368$$uhttps://zaguan.unizar.es/record/65321/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000065321 909CO $$ooai:zaguan.unizar.es:65321$$particulos$$pdriver
000065321 951__ $$a2019-07-09-12:39:14
000065321 980__ $$aARTICLE