000069477 001__ 69477
000069477 005__ 20220405150413.0
000069477 0247_ $$2doi$$a10.1158/2326-6074.TUMIMM16-B85
000069477 0248_ $$2sideral$$a104510
000069477 037__ $$aART-2017-104510
000069477 041__ $$aeng
000069477 100__ $$0(orcid)0000-0002-3796-4482$$aDel Amo, C.$$uUniversidad de Zaragoza
000069477 245__ $$aQuantification of sprouting angiogenesis under the effect of different growth factors involved in the tumor microenvironmen
000069477 260__ $$c2017
000069477 5060_ $$aAccess copy available to the general public$$fUnrestricted
000069477 5203_ $$aOne of the most important problems in tumor control is the management of metastatic process. Angiogenesis or the formation of new blood vessels from preexisting ones plays a crucial role in the expansion of the tumor by providing oxygen, nutrition and conduits for cancer cells to invade and metastasize new tissues¹. Abnormalities of growth factors (GFs) released such as PDGFs (Platelet Derived Growth Factor) could be involved in malignant human diseases2,3. Inflammation and cancer present similar mechanisms of development including angiogenesis or cell proliferation4. In order to know the effect on sprouting promotion of GFs existent in the tumor environment such as VEGF (Vascular Endothelial Growth Factor), PDGF, BMP2 (Bone Morphogenetic Protein 2) or TGF-ß (Transforming Growth Factor-ß), we have developed a microfluidic-based test based on devices designed by Farahat et al. (2012)5, which allows to the user the quantification of sprouting formation under the effect of these GFs. TGF-ß pathway involved in tumor progression in multiple human cancers, instigates phenotypical changes affecting to the cell growth, differentiation and migration6. Knowing the overexpression of GFs such as VEGF or BMP2 in tumors7,8, we aimed to compare its effect on endothelial cells in angiogenesis. Analyzing the promotion of sprout in normal conditions under GFs addition would be possible to determine which of these molecules could decrease or promote the advance of the endothelial cells. The results obtained in this work indicated that VEGF is the most important factor to enhance the angiogenic process while non-specific factors such as BMP2 or TGF-ß show a low effectiveness. In the case of PDGF, the negative effect of this molecule observed in our assays could be explained by the non-optimal balance of concentration. Furthermore, we are currently working to quantify the effect of fluid flow on the sprouting promotion.
000069477 536__ $$9info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751$$9info:eu-repo/grantAgreement/ES/MEC/FPU13-03194$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2015-64221-C2-1-R
000069477 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000069477 590__ $$a9.188$$b2017
000069477 591__ $$aONCOLOGY$$b16 / 223 = 0.072$$c2017$$dQ1$$eT1
000069477 591__ $$aIMMUNOLOGY$$b10 / 155 = 0.065$$c2017$$dQ1$$eT1
000069477 592__ $$a4.956$$b2017
000069477 593__ $$aImmunology$$c2017$$dQ1
000069477 593__ $$aCancer Research$$c2017$$dQ1
000069477 655_4 $$ainfo:eu-repo/semantics/conferenceObject$$vinfo:eu-repo/semantics/publishedVersion
000069477 700__ $$0(orcid)0000-0002-3784-1140$$aBorau, C.$$uUniversidad de Zaragoza
000069477 700__ $$0(orcid)0000-0002-0174-789X$$aAsín, J.$$uUniversidad de Zaragoza
000069477 700__ $$0(orcid)0000-0002-9864-7683$$aGarcía-Aznar, J. M.$$uUniversidad de Zaragoza
000069477 7102_ $$12007$$2265$$aUniversidad de Zaragoza$$bDpto. Métodos Estadísticos$$cÁrea Estadís. Investig. Opera.
000069477 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000069477 773__ $$g5, 3 (2017), B85$$pCancer immunol. res.$$tCancer immunology research$$x2326-6066
000069477 8564_ $$s254461$$uhttps://zaguan.unizar.es/record/69477/files/texto_completo.pdf$$yVersión publicada
000069477 8564_ $$s64787$$uhttps://zaguan.unizar.es/record/69477/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000069477 909CO $$ooai:zaguan.unizar.es:69477$$particulos$$pdriver
000069477 951__ $$a2022-04-05-14:35:32
000069477 980__ $$aARTICLE