000070053 001__ 70053
000070053 005__ 20190709135702.0
000070053 0247_ $$2doi$$a10.3390/mi9030129
000070053 0248_ $$2sideral$$a105453
000070053 037__ $$aART-2018-105453
000070053 041__ $$aeng
000070053 100__ $$aGonzález, I.
000070053 245__ $$aA label free disposable device for rapid isolation of rare tumor cells from blood by ultrasounds
000070053 260__ $$c2018
000070053 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070053 5203_ $$aThe use of blood samples as liquid biopsy is a label-free method for cancer diagnosis that offers benefits over traditional invasive biopsy techniques. Cell sorting by acoustic waves offers a means to separate rare cells from blood samples based on their physical properties in a label-free, contactless and biocompatible manner. Herein, we describe a flow-through separation approach that provides an efficient separation of tumor cells (TCs) from white blood cells (WBCs) in a microfluidic device, "THINUS-Chip" (Thin-Ultrasonic-Separator-Chip), actuated by ultrasounds. We introduce for the first time the concept of plate acoustic waves (PAW) applied to acoustophoresis as a new strategy. It lies in the geometrical chip design: different to other microseparators based on either bulk acoustic waves (BAW) or surface waves (SAW, SSAW and tSAW), it allows the use of polymeric materials without restrictions in the frequency of work. We demonstrate its ability to perform high-throughput isolation of TCs from WBCs, allowing a recovery rate of 84%±8% of TCs with a purity higher than 80% and combined viability of 85% at a flow rate of 80 µL/min (4.8 mL/h). The THINUS-Chip performs cell fractionation with low-cost manufacturing processes, opening the door to possible easy printing fabrication.
000070053 536__ $$9info:eu-repo/grantAgreement/ES/MINECO/BIO2011-30535-C04-01$$9info:eu-repo/grantAgreement/ES/MINECO/DPI2017-90147-R
000070053 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000070053 590__ $$a2.426$$b2018
000070053 591__ $$aINSTRUMENTS & INSTRUMENTATION$$b25 / 61 = 0.41$$c2018$$dQ2$$eT2
000070053 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b55 / 94 = 0.585$$c2018$$dQ3$$eT2
000070053 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070053 700__ $$aEarl, J.
000070053 700__ $$0(orcid)0000-0001-5376-4440$$aFernández, L.J.$$uUniversidad de Zaragoza
000070053 700__ $$aSainz, B.
000070053 700__ $$a, Jr.
000070053 700__ $$aPinto, A.
000070053 700__ $$0(orcid)0000-0003-0734-129X$$aMonge, R.
000070053 700__ $$aAlcalá, S.
000070053 700__ $$aCastillejo, A.
000070053 700__ $$aSoto, J.L.
000070053 700__ $$aCarrato, A.
000070053 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000070053 773__ $$g9, 3 (2018), 129 [15 pp]$$pMicromachines (Basel)$$tMicromachines (Basel)$$x2072-666X
000070053 8564_ $$s743517$$uhttp://zaguan.unizar.es/record/70053/files/texto_completo.pdf$$yVersión publicada
000070053 8564_ $$s107238$$uhttp://zaguan.unizar.es/record/70053/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070053 909CO $$ooai:zaguan.unizar.es:70053$$particulos$$pdriver
000070053 951__ $$a2019-07-09-12:49:36
000070053 980__ $$aARTICLE