A label free disposable device for rapid isolation of rare tumor cells from blood by ultrasounds
Resumen: The use of blood samples as liquid biopsy is a label-free method for cancer diagnosis that offers benefits over traditional invasive biopsy techniques. Cell sorting by acoustic waves offers a means to separate rare cells from blood samples based on their physical properties in a label-free, contactless and biocompatible manner. Herein, we describe a flow-through separation approach that provides an efficient separation of tumor cells (TCs) from white blood cells (WBCs) in a microfluidic device, "THINUS-Chip" (Thin-Ultrasonic-Separator-Chip), actuated by ultrasounds. We introduce for the first time the concept of plate acoustic waves (PAW) applied to acoustophoresis as a new strategy. It lies in the geometrical chip design: different to other microseparators based on either bulk acoustic waves (BAW) or surface waves (SAW, SSAW and tSAW), it allows the use of polymeric materials without restrictions in the frequency of work. We demonstrate its ability to perform high-throughput isolation of TCs from WBCs, allowing a recovery rate of 84%±8% of TCs with a purity higher than 80% and combined viability of 85% at a flow rate of 80 µL/min (4.8 mL/h). The THINUS-Chip performs cell fractionation with low-cost manufacturing processes, opening the door to possible easy printing fabrication.
Idioma: Inglés
DOI: 10.3390/mi9030129
Año: 2018
Publicado en: Micromachines (Basel) 9, 3 (2018), 129 [15 pp]
ISSN: 2072-666X

Factor impacto JCR: 2.426 (2018)
Categ. JCR: INSTRUMENTS & INSTRUMENTATION rank: 25 / 61 = 0.41 (2018) - Q2 - T2
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 55 / 94 = 0.585 (2018) - Q3 - T2

Factor impacto SCIMAGO:

Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2011-30535-C04-01
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2017-90147-R
Tipo y forma: Article (Published version)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)

Creative Commons You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.


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