000070997 001__ 70997
000070997 005__ 20210108084705.0
000070997 0247_ $$2doi$$a10.1002/jgm.2964
000070997 0248_ $$2sideral$$a101058
000070997 037__ $$aART-2017-101058
000070997 041__ $$aeng
000070997 100__ $$0(orcid)0000-0003-4891-105X$$aEncabo-Berzosa, M.M.$$uUniversidad de Zaragoza
000070997 245__ $$aPolymer functionalized gold nanoparticles as nonviral gene delivery reagents
000070997 260__ $$c2017
000070997 5060_ $$aAccess copy available to the general public$$fUnrestricted
000070997 5203_ $$aBackground: In the present study, we investigated the ability of polyethylene glycol (PEG) functionalized gold nanoparticles to function as nonviral vectors in the transfection of different cell lines, comparing them with commercial lipoplexes.
Methods: Positively-charged gold nanoparticles were synthesized using polyethylenimine (PEI) as a reducing and stabilizer agent and its cytotoxicity was reduced by its functionalization with PEG. We bound the nanoparticles to three plasmids with different sizes (4–40 kpb). Vector internalization was evaluated by confocal and electronic microscopy. Its transfection efficacy was studied by fluorescence microscopy and flow cytometry. The application of the resulting vector in gene therapy was evaluated indirectly using ganciclovir in HeLa cells transfected to express the herpes virus thymidine kinase.
Results: An appropriate ratio between the nitrogen from the PEI and the phosphorous from the phosphate groups of the DNA, together with a reduced size and an elevated electrokinetic potential, are responsible for an increased nanoparticle internalization and enhanced protein expression when carrying plasmids of up to 40 kbp (plasmid size close to the limit of the DNA-carrying capacity of viral vectors). Compared to a commercial transfection reagent, an equal or even higher expression of reporter genes (on HeLa and Hek293t) and a suicide effect on HeLa cells transfected with the herpes virus thymidine kinase gene were observed when using this novel nanoparticulated vector.
Conclusions: Nonviral vectors based on gold nanoparticles covalently coupled with PEG and PEI can be used as efficient transfection reagents showing expression levels that are the same or greater than those obtained with commercially available lipoplexes.
000070997 536__ $$9info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM
000070997 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000070997 590__ $$a1.647$$b2017
000070997 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b110 / 160 = 0.688$$c2017$$dQ3$$eT3
000070997 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b101 / 133 = 0.759$$c2017$$dQ4$$eT3
000070997 591__ $$aGENETICS & HEREDITY$$b130 / 171 = 0.76$$c2017$$dQ4$$eT3
000070997 592__ $$a0.493$$b2017
000070997 593__ $$aDrug Discovery$$c2017$$dQ3
000070997 593__ $$aMolecular Medicine$$c2017$$dQ3
000070997 593__ $$aMolecular Biology$$c2017$$dQ4
000070997 593__ $$aGenetics$$c2017$$dQ4
000070997 593__ $$aGenetics (clinical)$$c2017$$dQ4
000070997 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000070997 700__ $$0(orcid)0000-0001-8762-5457$$aSancho-Albero, M.$$uUniversidad de Zaragoza
000070997 700__ $$0(orcid)0000-0002-6873-5244$$aSebastian, V.$$uUniversidad de Zaragoza
000070997 700__ $$0(orcid)0000-0002-2966-9088$$aIrusta, S.$$uUniversidad de Zaragoza
000070997 700__ $$0(orcid)0000-0003-3165-0156$$aArruebo, M.$$uUniversidad de Zaragoza
000070997 700__ $$0(orcid)0000-0002-8701-9745$$aSantamaria, J.$$uUniversidad de Zaragoza
000070997 700__ $$0(orcid)0000-0003-4160-9720$$aMartín Duque, P.
000070997 7102_ $$15005$$2555$$aUniversidad de Zaragoza$$bDpto. Ing.Quím.Tecnol.Med.Amb.$$cÁrea Ingeniería Química
000070997 773__ $$g19, 6-7 (2017), e2964 [12 pp]$$pJ. gene med.$$tJOURNAL OF GENE MEDICINE$$x1099-498X
000070997 8564_ $$s816627$$uhttps://zaguan.unizar.es/record/70997/files/texto_completo.pdf$$yVersión publicada
000070997 8564_ $$s99565$$uhttps://zaguan.unizar.es/record/70997/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000070997 909CO $$ooai:zaguan.unizar.es:70997$$particulos$$pdriver
000070997 951__ $$a2021-01-08-08:03:57
000070997 980__ $$aARTICLE