A perspective on structural and mechanistic aspects of protein O-fucosylation

Lira-Navarrete, E. ; Hurtado-Guerrero, R. (Universidad de Zaragoza)

Resumen: Protein O-fucosylation is an important post-translational modification (PTM) found in cysteine-rich repeats in proteins. Protein O-fucosyltransferases 1 and 2 (PoFUT1 and PoFUT2) are the enzymes responsible for this PTM and selectively glycosylate specific residues in epidermal growth factor-like (EGF) repeats and thrombospondin type I repeats (TSRs), respectively.Within the past six years, crystal structures of both enzymes have been reported, revealing important information on how they recognize protein substrates and achieve catalysis. Here, the structural information available today is summarized and how PoFUT1 and PoFUT2 employ different catalytic mechanisms is discussed.

Idioma: Inglés

DOI: 10.1107/S2053230X18004788

Año: 2018

Publicado en: Acta Crystallographica Section F-Structural Biology Communications 74, 8 (2018), 443-450
ISSN: 2053-230X

Factor impacto SCIMAGO: 0.701 - Biochemistry (Q2) - Biophysics (Q2) - Structural Biology (Q2) - Genetics (Q2) - Medicine (miscellaneous) (Q2) - Condensed Matter Physics (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B89
Financiación: info:eu-repo/grantAgreement/EC/FP7/609409/EU/Supporting transnational mobility within the European life sciences by cofunding of the EMBO Fellowship Programme/LTFCOFUND2013

Tipo y forma: Review (Published version)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)