Carbapenem-resistant Citrobacter spp. isolated in Spain from 2013 to 2015 produced a variety of carbapenemases including VIM-1, OXA-48, KPC-2, NDM-1 and VIM-2

Arana, D.M. ; Ortega, A. ; González-Barberá, E. ; Lara, N. ; Bautista, V. ; Gómez-Ruíz, D. ; Sáez, D. ; Fernández-Romero, S. ; Aracil, B. ; Pérez-Vázquez, M. ; Campos, J. ; Oteo, J. ; Gómez-Alfaro, I. ; Aznar, J.E. ; Cercenado, E. ; López-Urrutia, L. ; García-Picazo, L. ; López-Calleja, A.I. ; Sánchez-Romero, I. ; Zamarrón-Fuertes, P. ; Leiva, J. ; Alós, J.I. ; Solís, S. ; de Miguel, M.D. ; Hernández, B. ; Romanyk, J. ; Delgado-Iribarren, A. ; Fernández, E.O. ; Trujillo, G. ; Torroba, L. ; Hernández Almaraz, J.L. ; Remacha Esteras, M.A. ; Salso, S. ; Gil, Y. ; Rodríguez-Conde, I. ; Alarcón, T.
Carbapenem-resistant Citrobacter spp. isolated in Spain from 2013 to 2015 produced a variety of carbapenemases including VIM-1, OXA-48, KPC-2, NDM-1 and VIM-2
Resumen: Objectives: There is little information about carbapenemase-producing (CP) Citrobacter spp.We studied the molecular epidemiology and microbiological features of CP Citrobacter spp. isolates collected in Spain (2013-15).
Methods: In total, 119 isolates suspected of being CP by the EUCAST screening cut-off values were analysed. Carbapenemases and ESBLs were characterized using PCR and sequencing. The genetic relationship among Citrobacter freundii isolates was studied by PFGE.
Results: Of the 119 isolates, 63 (52.9%) produced carbapenemases, of which 37 (58.7%) produced VIM-1, 20 (31.7%) produced OXA-48, 12 (19%) produced KPC-2, 2 (3.2%) produced NDM-1 and 1 (1.6%) produced VIM- 2; 9 C. freundii isolates co-produced VIM-1 plus OXA-48. Fourteen isolates (22.2%) also carried ESBLs: 8 CTX-M-9 plus SHV-12, 2 CTX-M-9, 2 SHV-12 and 2 CTX-M-15. Fifty-seven isolates (90.5%) were C. freundii, 4 (6.3%) were Citrobacter koseri, 1 (1.6%) was Citrobacter amalonaticus and 1 (1.6%) was Citrobacter braakii. By EUCAST breakpoints, eight (12.7%) of the CP isolates were susceptible to the four carbapenems tested. In the 53 CP C. freundii analysed by PFGE, a total of 44 different band patterns were observed. Four PFGE clusters were identified: cluster 1 included eight isolates co-producing VIM-1 and OXA-48; blaVIM-1 was carried in a class 1 integron (intI-blaVIM-1 - aacA4-dfrB1-aadA1-catB2-qacE¿1/sul1) and blaOXA-48 was carried in a Tn1999.2 transposon.
Conclusions: We observed the clonal and polyclonal spread of CP Citrobacter spp. across several Spanish geographical areas. Four species of Citrobacter spp. produced up to five carbapenemase types, including coproduction of VIM-1 plus OXA-48. Some CP Citrobacter spp. isolates were susceptible to the four carbapenems tested, a finding with potential clinical implications.

Idioma: Inglés
DOI: 10.1093/jac/dkx325
Año: 2017
Publicado en: Journal of Antimicrobial Chemotherapy 72, 12 (2017), 3283-3287
ISSN: 0305-7453

Factor impacto JCR: 5.217 (2017)
Categ. JCR: INFECTIOUS DISEASES rank: 8 / 88 = 0.091 (2017) - Q1 - T1
Categ. JCR: PHARMACOLOGY & PHARMACY rank: 19 / 261 = 0.073 (2017) - Q1 - T1
Categ. JCR: MICROBIOLOGY rank: 19 / 125 = 0.152 (2017) - Q1 - T1

Factor impacto SCIMAGO: 2.419 - Infectious Diseases (Q1) - Pharmacology (medical) (Q1) - Pharmacology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO-ISCIII/PY1135-16
Tipo y forma: Artículo (Versión definitiva)

Derechos Reservados Derechos reservados por el editor de la revista


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