000078238 001__ 78238
000078238 005__ 20191122145056.0
000078238 0247_ $$2doi$$a10.1016/j.jprot.2017.08.018
000078238 0248_ $$2sideral$$a101851
000078238 037__ $$aART-2018-101851
000078238 041__ $$aeng
000078238 100__ $$0(orcid)0000-0003-1892-5063$$aCeballos-Laita, Laura
000078238 245__ $$aEffects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses
000078238 260__ $$c2018
000078238 5060_ $$aAccess copy available to the general public$$fUnrestricted
000078238 5203_ $$aThe aim of this work was to study the effects of Fe and Mn deficiencies on the xylem sap proteome of tomato using a shotgun proteomic approach, with the final goal of elucidating plant response mechanisms to these stresses. This approach yielded 643 proteins reliably identified and quantified with 70% of them predicted as secretory. Iron and Mn deficiencies caused statistically significant and biologically relevant abundance changes in 119 and 118 xylem sap proteins, respectively. In both deficiencies, metabolic pathways most affected were protein metabolism, stress/oxidoreductases and cell wall modifications. First, results suggest that Fe deficiency elicited more stress responses than Mn deficiency, based on the changes in oxidative and proteolytic enzymes. Second, both nutrient deficiencies affect the secondary cell wall metabolism, with changes in Fe deficiency occurring via peroxidase activity, and in Mn deficiency involving peroxidase, Cu-oxidase and fasciclin-like arabinogalactan proteins. Third, the primary cell wall metabolism was affected by both nutrient deficiencies, with changes following opposite directions as judged from the abundances of several glycoside-hydrolases with endo-glycolytic activities and pectin esterases. Fourth, signaling pathways via xylem involving CLE and/or lipids as well as changes in phosphorylation and N-glycosylation also play a role in the responses to these stresses. Biological significance In spite of being essential for the delivery of nutrients to the shoots, our knowledge of xylem responses to nutrient deficiencies is very limited. The present work applies a shotgun proteomic approach to unravel the effects of Fe and Mn deficiencies on the xylem sap proteome. Overall, Fe deficiency seems to elicit more stress in the xylem sap proteome than Mn deficiency, based on the changes measured in proteolytic and oxido-reductase proteins, whereas both nutrients exert modifications in the composition of the primary and secondary cell wall. Cell wall modifications could affect the mechanical and permeability properties of the xylem sap vessels, and therefore ultimately affect solute transport and distribution to the leaves. Results also suggest that signaling cascades involving lipid and peptides might play a role in nutrient stress signaling and pinpoint interesting candidates for future studies. Finally, both nutrient deficiencies seem to affect phosphorylation and glycosylation processes, again following an opposite pattern.
000078238 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A03$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/AGL2013-42175-R
000078238 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000078238 590__ $$a3.537$$b2018
000078238 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b18 / 78 = 0.231$$c2018$$dQ1$$eT1
000078238 592__ $$a1.15$$b2018
000078238 593__ $$aBiophysics$$c2018$$dQ1
000078238 593__ $$aBiochemistry$$c2018$$dQ1
000078238 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000078238 700__ $$aGutierrez-Carbonell, Elaín
000078238 700__ $$aTakahashi, Daisuke
000078238 700__ $$aAbadía, Anunciación
000078238 700__ $$aUemura, Matsuo
000078238 700__ $$aAbadía, Javier
000078238 700__ $$aLópez-Millán, Ana Flor
000078238 773__ $$g170 (2018), 117-129$$pJ. proteomics$$tJOURNAL OF PROTEOMICS$$x1874-3919
000078238 8564_ $$s603641$$uhttps://zaguan.unizar.es/record/78238/files/texto_completo.pdf$$yPostprint
000078238 8564_ $$s52079$$uhttps://zaguan.unizar.es/record/78238/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000078238 909CO $$ooai:zaguan.unizar.es:78238$$particulos$$pdriver
000078238 951__ $$a2019-11-22-14:46:10
000078238 980__ $$aARTICLE