000078996 001__ 78996 000078996 005__ 20200716101449.0 000078996 0247_ $$2doi$$a10.1371/journal.pone.0214004 000078996 0248_ $$2sideral$$a111463 000078996 037__ $$aART-2019-111463 000078996 041__ $$aeng 000078996 100__ $$aCorrea, S. 000078996 245__ $$aDesign of stable magnetic hybrid nanoparticles of Si-entrapped HRP 000078996 260__ $$c2019 000078996 5060_ $$aAccess copy available to the general public$$fUnrestricted 000078996 5203_ $$aHybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silica (Si) nanoparticles entrapping both Horseradish Peroxidase (HRP) (EC 1.11.1.7) and magnetic nanoparticles (MNPs). We have demonstrated that tailoring of the synthetic reagents and post immobilization treatments greatly impacted physical and biocatalytic properties such as an unprecedented ~280 times increase in the half-life time in thermal stability experiments. The optimized nanohybrid biocatalyst that showed superparamagnetic behaviour, was effective in the batch conversion of indole-3-acetic acid, a prodrug used in Direct Enzyme Prodrug Therapy (DEPT). Our system, that was not cytotoxic per se, showed enhanced cytotoxic activity in the presence of the prodrug towards HCT-116, a colorectal cancer cell line. The strategy developed proved to be effective in obtaining a stabilized nanobiocatalyst combining three different organic/inorganic materials with potential in DEPT and other biotechnological applications. 000078996 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000078996 590__ $$a2.74$$b2019 000078996 591__ $$aMULTIDISCIPLINARY SCIENCES$$b27 / 71 = 0.38$$c2019$$dQ2$$eT2 000078996 592__ $$a1.023$$b2019 000078996 593__ $$aMultidisciplinary$$c2019$$dQ1 000078996 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000078996 700__ $$aPuertas, S. 000078996 700__ $$0(orcid)0000-0003-2366-3598$$aGutiérrez, L.$$uUniversidad de Zaragoza 000078996 700__ $$aAsín, L. 000078996 700__ $$0(orcid)0000-0003-1081-8482$$aDe La Fuente, J.M. 000078996 700__ $$0(orcid)0000-0001-6170-4237$$aGrazú, V.$$uUniversidad de Zaragoza 000078996 700__ $$aBetancor, L. 000078996 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica 000078996 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica 000078996 773__ $$g14, 4 (2019), e0214004[19 pp]$$pPLoS One$$tPLoS ONE$$x1932-6203 000078996 8564_ $$s969209$$uhttps://zaguan.unizar.es/record/78996/files/texto_completo.pdf$$yVersión publicada 000078996 8564_ $$s98323$$uhttps://zaguan.unizar.es/record/78996/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000078996 909CO $$ooai:zaguan.unizar.es:78996$$particulos$$pdriver 000078996 951__ $$a2020-07-16-09:05:00 000078996 980__ $$aARTICLE