Presence of Clostridium difficile in pig faecal samples and wild animal species associated with pig farms

Andrés-Lasheras, S. ; Bolea, R. (Universidad de Zaragoza) ; Mainar-Jaime, R. C. (Universidad de Zaragoza) ; Kuijper, E. ; Sevilla, E. (Universidad de Zaragoza) ; Martín-Burriel, I. (Universidad de Zaragoza) ; Chirino-Trejo, M.

Resumen: Aims: to determine the presence of Clostridium difficile on fattening pig farms in north-eastern Spain. Methods and results: Twenty-seven farms were sampled. Pools of pig faecal samples (n = 210), samples of intestinal content from common farm pest species (n = 95) and environment-related samples (n = 93) were collected. Isolates were tested for toxin genes of C. difficile, and typed by PCR-ribotyping and toxinotyping. The minimal inhibitory concentrations of six antimicrobial agents were determined using Etest. Thirty-four isolates were obtained from 12 farms, and 30 (88·2%) had toxin genes. Seven ribotypes were identified. Ribotype 078 and its variant 126 were predominant (52·9%). The same ribotypes were isolated from different animal species on the same farm. None of the isolates were resistant to metronidazole or vancomycin. Conclusions: Clostridium difficile was common within the pig farm environment. Most of the positive samples came from pest species or were pest-related environmental samples. Significance and Impact of the Study: Pest species were colonized with toxigenic and antimicrobial-resistant C. difficile strains of the same ribotypes that are found in humans and pigs. Rodents and pigeons may transmit toxigenic and antimicrobial-resistant C. difficile strains that are of the same ribotypes as those occuring in humans.

Idioma: Inglés

DOI: 10.1111/jam.13343

Año: 2017

Publicado en: Journal of Applied Microbiology 122, 2 (2017), 462-472 [27 p.]
ISSN: 1364-5072

Factor impacto JCR: 2.16 (2017)

Categ. JCR: MICROBIOLOGY rank: 79 / 125 = 0.632 (2017) - Q3 - T2
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 82 / 160 = 0.512 (2017) - Q3 - T2

Factor impacto SCIMAGO: 0.795 - Applied Microbiology and Biotechnology (Q2) - Medicine (miscellaneous) (Q2) - Biotechnology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/INIA/FAU2008-16

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)