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000008587 001__ 8587
000008587 037__ $$aTAZ-PFC-2012-430
000008587 041__ $$aeng
000008587 1001_ $$aViamonte Dominguez, Juan
000008587 24500 $$aDetermination of aspartame in soft drinks using HPLC
000008587 260__ $$aZaragoza$$bUniversidad de Zaragoza$$c2012
000008587 506__ $$aby-nc-sa$$bCreative Commons$$c3.0$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/
000008587 500__ $$aThis report describes the results of the project on the 4th semester at the Institute of Chemistry, Biotechnology and Environmental Technology (KBM) at the Technical Faculty of the University of Southern Denmark.
000008587 520__ $$aThe aim of this project is to determine the levels of aspartame in various cola products using High Pressure Liquid Chromatography (HPLC). Four different cola brands are examined, Coca-Cola Zero, Coca-Cola Light, Pepsi Max and Harboe Cola Minus. There is written a section of theory about aspartame, the environment and legislation for the substance. Additionally, there is made a theoretical section on HPLC and statistics. In the laboratory, a method for the examination of the aspartame in the coke product is created. This method is used to collect data for the various fabricates which are examined using various statistical parameters. In method development, it is found that aspartame can be separated completely from the other ingredients of the cola products. Aspartame content is verified by means of a certified standard substance. In the laboratory an Agilent HPLC is used with a C-18 column, 150 mm long and a particle diameter of 5 micrometers. The method uses a gradient program of acetonitrile and 22 mM phosphate buffer pH 2.5. The method is confirmed by statistical calculations that show that the data for the same cola is both repeatable and reproducible. Through tests of newer colas of the same brands, it is shown that the concentration of aspartame in the same brand vary significantly from bottle to bottle. The recovery of the method is close to 100 %, which is extremely essential for the validation of the method.
000008587 521__ $$aIngeniero Técnico Industrial (Esp. Química Industrial)
000008587 540__ $$aDerechos regulados por licencia Creative Commons
000008587 6531_ $$adetermination
000008587 6531_ $$aaspartame
000008587 6531_ $$asoft drinks
000008587 6531_ $$ahplc
000008587 6531_ $$aartificial
000008587 6531_ $$asweetener
000008587 700__ $$aRubio Navarro, Carlos Enrique$$edir.
000008587 7102_ $$aUniversidad de Zaragoza$$bQuímica Analítica$$cQuímica Analítica
000008587 8560_ $$f510242@celes.unizar.es
000008587 8564_ $$s1405053$$uhttp://zaguan.unizar.es/TAZ/EINA/2012/8587/TAZ-PFC-2012-430.pdf$$zMemoria (eng)
000008587 909CO $$ooai:zaguan.unizar.es:8587$$ppublic
000008587 909CO $$pTAZ
000008587 950__ $$a
000008587 980__ $$aTAZ$$bPFC$$cEINA
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