8587 Zaragoza Universidad de Zaragoza 2012 by-nc-sa Creative Commons 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ Derechos regulados por licencia Creative Commons 510242@celes.unizar.es determination aspartame soft drinks hplc artificial sweetener TAZ PFC EINA Rubio Navarro, Carlos Enrique dir. Universidad de Zaragoza Química Analítica Química Analítica Determination of aspartame in soft drinks using HPLC The aim of this project is to determine the levels of aspartame in various cola products using High Pressure Liquid Chromatography (HPLC). Four different cola brands are examined, Coca-Cola Zero, Coca-Cola Light, Pepsi Max and Harboe Cola Minus. There is written a section of theory about aspartame, the environment and legislation for the substance. Additionally, there is made a theoretical section on HPLC and statistics. In the laboratory, a method for the examination of the aspartame in the coke product is created. This method is used to collect data for the various fabricates which are examined using various statistical parameters. In method development, it is found that aspartame can be separated completely from the other ingredients of the cola products. Aspartame content is verified by means of a certified standard substance. In the laboratory an Agilent HPLC is used with a C-18 column, 150 mm long and a particle diameter of 5 micrometers. The method uses a gradient program of acetonitrile and 22 mM phosphate buffer pH 2.5. The method is confirmed by statistical calculations that show that the data for the same cola is both repeatable and reproducible. Through tests of newer colas of the same brands, it is shown that the concentration of aspartame in the same brand vary significantly from bottle to bottle. The recovery of the method is close to 100 %, which is extremely essential for the validation of the method. eng Viamonte Dominguez, Juan TAZ-PFC-2012-430 This report describes the results of the project on the 4th semester at the Institute of Chemistry, Biotechnology and Environmental Technology (KBM) at the Technical Faculty of the University of Southern Denmark. Ingeniero Técnico Industrial (Esp. Química Industrial) oai:zaguan.unizar.es:8587 TAZ public http://zaguan.unizar.es/TAZ/EINA/2012/8587/TAZ-PFC-2012-430.pdf 1405053 Memoria (eng)