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oai:zaguan.unizar.es:8587 2015-04-29
eng Viamonte Dominguez, Juan Rubio Navarro, Carlos Enrique Determination of aspartame in soft drinks using HPLC http://zaguan.unizar.es/record/8587/files/TAZ-PFC-2012-430.pdf The aim of this project is to determine the levels of aspartame in various cola products using High Pressure Liquid Chromatography (HPLC). Four different cola brands are examined, Coca-Cola Zero, Coca-Cola Light, Pepsi Max and Harboe Cola Minus. There is written a section of theory about aspartame, the environment and legislation for the substance. Additionally, there is made a theoretical section on HPLC and statistics. In the laboratory, a method for the examination of the aspartame in the coke product is created. This method is used to collect data for the various fabricates which are examined using various statistical parameters. In method development, it is found that aspartame can be separated completely from the other ingredients of the cola products. Aspartame content is verified by means of a certified standard substance. In the laboratory an Agilent HPLC is used with a C-18 column, 150 mm long and a particle diameter of 5 micrometers. The method uses a gradient program of acetonitrile and 22 mM phosphate buffer pH 2.5. The method is confirmed by statistical calculations that show that the data for the same cola is both repeatable and reproducible. Through tests of newer colas of the same brands, it is shown that the concentration of aspartame in the same brand vary significantly from bottle to bottle. The recovery of the method is close to 100 %, which is extremely essential for the validation of the method. 2014-11-27
8587 20150429092417.0 TAZ-PFC-2012-430 eng Viamonte Dominguez, Juan Determination of aspartame in soft drinks using HPLC Zaragoza Universidad de Zaragoza 2012 by-nc-sa Creative Commons 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ This report describes the results of the project on the 4th semester at the Institute of Chemistry, Biotechnology and Environmental Technology (KBM) at the Technical Faculty of the University of Southern Denmark. The aim of this project is to determine the levels of aspartame in various cola products using High Pressure Liquid Chromatography (HPLC). Four different cola brands are examined, Coca-Cola Zero, Coca-Cola Light, Pepsi Max and Harboe Cola Minus. There is written a section of theory about aspartame, the environment and legislation for the substance. Additionally, there is made a theoretical section on HPLC and statistics. In the laboratory, a method for the examination of the aspartame in the coke product is created. This method is used to collect data for the various fabricates which are examined using various statistical parameters. In method development, it is found that aspartame can be separated completely from the other ingredients of the cola products. Aspartame content is verified by means of a certified standard substance. In the laboratory an Agilent HPLC is used with a C-18 column, 150 mm long and a particle diameter of 5 micrometers. The method uses a gradient program of acetonitrile and 22 mM phosphate buffer pH 2.5. The method is confirmed by statistical calculations that show that the data for the same cola is both repeatable and reproducible. Through tests of newer colas of the same brands, it is shown that the concentration of aspartame in the same brand vary significantly from bottle to bottle. The recovery of the method is close to 100 %, which is extremely essential for the validation of the method. Ingeniero Técnico Industrial (Esp. Química Industrial) Derechos regulados por licencia Creative Commons determination aspartame soft drinks hplc artificial sweetener Rubio Navarro, Carlos Enrique dir. Universidad de Zaragoza Química Analítica Química Analítica 510242@celes.unizar.es 1405053 http://zaguan.unizar.es/record/8587/files/TAZ-PFC-2012-430.pdf Memoria (eng) Memoria (eng) oai:zaguan.unizar.es:8587 driver proyectos-fin-carrera TAZ PFC EINA URI http://zaguan.unizar.es/record/8587 SUPPORTED 0 MD5 http://zaguan.unizar.es/record/8587/files/TAZ-PFC-2012-430.md5 0 image/x.djvu 6 http://djvu.sourceforge.net/abstract.html DJVU/6 Profile information Lizardtech Document Express Enterprise 5.1 0 URI http://zaguan.unizar.es/record/8587/files/TAZ-PFC-2012-430.pdf disk Minimum View Print Visualization of DJVU requires specific software, like DjVu Browser Plugin URI http://creativecommons.org/licenses/by-nc/3.0 URI http://creativecommons.org/licenses/by-nc/3.0 license URI http://creativecommons.org/licenses/by-nc/3.0 You are free to adapt, copy, transmite or distribute the work under the following conditions: (1) You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). (2) You may not use this work for commercial purposes (3) For any reuse or distribution, you must make clear to others the license terms of this work (4) Any of the above conditions can be waived if you get permission from the copyright holder (5) Nothing in this license impairs or restricts the author's moral rights This object is licensed under Creative Common Attribution-NonCommercial 3.0 (further details: http://creativecommons.org/licenses/by-nc/3.0/). Universidad de Zaragoza Automatizacion de Bibliotecas Edif. Matematicas, Pedro Cerbuna 12, 50009 Zaragoza auto.buz@unizar.es