000087548 001__ 87548
000087548 005__ 20200716101515.0
000087548 0247_ $$2doi$$a10.1016/j.cryobiol.2019.01.009
000087548 0248_ $$2sideral$$a110408
000087548 037__ $$aART-2019-110408
000087548 041__ $$aeng
000087548 100__ $$aÁlvarez, C.
000087548 245__ $$aA preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen
000087548 260__ $$c2019
000087548 5060_ $$aAccess copy available to the general public$$fUnrestricted
000087548 5203_ $$aSperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Ejaculates were collected from five fertile Donkeys. Sperm samples were pooled, diluted and cryopreserved into three experimental extender groups: BotuCrio®, lactose-extender supplemented with egg yolk (20%) and lactose-extender supplemented with jenny colostrum (20%). The results demonstrated that lactose-jenny colostrum samples displayed significantly higher values in almost all parameters evaluated (p < 0.05) compared with the other two extenders after thawing (BotuCrio® and lactose-egg yolk based extender, respectively) –Total Motility, Viability, HOS test, VCL, VSL and VAP. Acrosome status, LIN, STR and WOB despite showing lower values, none of them were statistically significant (p > 0.05). In conclusion, the extender containing jenny colostrum can be successfully used for donkey semen crypreservation and could effectively improve donkey sperm qualities after freezing -thawing.
000087548 536__ $$9info:eu-repo/grantAgreement/ES/DGA/FSE
000087548 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000087548 590__ $$a2.283$$b2019
000087548 591__ $$aBIOLOGY$$b40 / 93 = 0.43$$c2019$$dQ2$$eT2
000087548 591__ $$aPHYSIOLOGY$$b44 / 81 = 0.543$$c2019$$dQ3$$eT2
000087548 592__ $$a0.696$$b2019
000087548 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2019$$dQ1
000087548 593__ $$aMedicine (miscellaneous)$$c2019$$dQ2
000087548 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2019$$dQ2
000087548 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000087548 700__ $$0(orcid)0000-0002-7857-9732$$aLuño, V.$$uUniversidad de Zaragoza
000087548 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, N.$$uUniversidad de Zaragoza
000087548 700__ $$0(orcid)0000-0002-0797-1841$$aGil, L.$$uUniversidad de Zaragoza
000087548 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000087548 773__ $$g87 (2019), 110-114$$pCryobiology$$tCryobiology$$x0011-2240
000087548 8564_ $$s163702$$uhttps://zaguan.unizar.es/record/87548/files/texto_completo.pdf$$yPostprint
000087548 8564_ $$s42624$$uhttps://zaguan.unizar.es/record/87548/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000087548 909CO $$ooai:zaguan.unizar.es:87548$$particulos$$pdriver
000087548 951__ $$a2020-07-16-09:22:46
000087548 980__ $$aARTICLE